江苏大学学报（自然科学版）2017，Vol.38Issue(5)：596-602,7.DOI:10.3969/j.issn.1671-7775.2017.05.016
时间分辨免疫法检测镇江市内水样中酞酸二丁酯
Time-resolved fluorescence immunoassay for determination ofdibutyl phthalate in water samples of Zhenjiang city
摘要
Abstract
As an endocrine disruptor, dibutyl phthalate (DBP) has adverse effects on environment and human health.Using a polyclonal antibody of DBP, a sensitive indirectly competitive time-resolved fluorescence immunoassay(TRFIA) was proposed, and several parameters were investigated.DBP concentration in the rivers of Zhenjiang was determined, and the ecological risk was assessed by risk quotients.The results show that under the optimized conditions, the 50% inhibition concentration (IC50) value of TRFIA is 35.8 ng·mL-1 with limit detection value of 2.4 ng·mL-1 in the detection range from 4.7 to 272.6 ng·mL-1.The developed method shows high selectivity with cross-reactivities less than 4.65% to seven structurally related phthalate esters.The recoveries are from 79.85% to 119.20%, and the reproducibilities of intra-assay CV are from 5.54% to 9.93% with reproducibilities of inter-assay CV from 7.27% to 14.01%.The detection rate of DBP in the rivers of Zhenjiang city is 100%, and the concentration ranges from 5.89 to 94.57 ng·mL-1.There exists high ecological risk for DBP distribution in rivers.关键词
酞酸二丁酯/多克隆抗体/荧光免疫分析/时间分辨/风险熵法Key words
DBP/polyclonal antibody/fluorescence immunoassay/time-resolved/risk quotient分类
资源环境引用本文复制引用
朱方,柴帅,宾金坤,杜道林..时间分辨免疫法检测镇江市内水样中酞酸二丁酯[J].江苏大学学报（自然科学版）,2017,38(5):596-602,7.基金项目
国家自然科学基金资助项目(31570414) （31570414）
江苏大学大学生科研立项项目(15A309) （15A309）
