青岛大学学报（自然科学版）2017，Vol.30Issue(2)：39-44,6.DOI:10.3969/j.issn.1006-1037.2017.05.09
HCMV感染对下调ATF5的人胶质瘤U251细胞干性的影响
The Effect of HCMV Infection on the Stemness of Down-regulated ATF5 U251 Cells
摘要
Abstract
To study the effect of HCMV infection on the expression of CD133, cell proliferation and clone formation of U251 cells, which were down regulated the ATF5 expression.The changes of IE, CD133 gene and protein expression levels of HCMV (MOI=1) infected U251 cells were detected by fluorescence quantitative PCR and Western-blot methods.The results showed that the expression of CD133 increased significantly with the increase of IE expression in U251 cells.Silencing ATF5 cell line siATF5-U251 was successfully constructed, and HCMV infection reduced the cell proliferation activity, and decreased the colony formation rate.The results of fluorescence quantitative PCR and Western-blot showed that HCMV infection significantly inhibited the expression of CD133 in siATF5-U251 cells.Therefore, inhibiting the expression of ATF5 may be a new target for prevention and treatment of malignant glioma.关键词
人巨细胞病毒/人胶质瘤U251细胞/CD133/转录激活因子5Key words
Human cytomegalovirus/U251 glioma cell/CD133/transcription factor 5分类
生物科学引用本文复制引用
王心慧,钱冬萌,王斌,朱文华..HCMV感染对下调ATF5的人胶质瘤U251细胞干性的影响[J].青岛大学学报（自然科学版）,2017,30(2):39-44,6.基金项目
国家自然科学基金(批准号:81471958)资助. （批准号:81471958）
