色谱2017，Vol.35Issue(8)：855-859,5.DOI:10.3724/SP.J.1123.2017.03001
非对称场流分离技术在蛋黄浆质低密度脂蛋白粒径表征中的应用
Application of asymmetrical flow field-flow fractionation for size characterization of low density lipoprotein in egg yolk plasma
摘要
Abstract
Home-made asymmetrical flow field-flow fractionation (AF4) system, online coupled with ultraviolet/visible (UV/Vis) detector was employed for the separation and size characterization of low density lipoprotein (LDL) in egg yolk plasma.At close to natural condition of egg yolk, the effects of cross flow rate, sample loading, and type of membrane on the size distribution of LDL were investigated.Under the optimal operation conditions, AF4-UV/Vis provides the size distribution of LDL.Moreover, the precision of AF4-UV/Vis method proposed in this work for the analysis of LDL in egg yolk plasma was evaluated.The intra-day precisions were 1.3% and 1.9% (n=7) and the inter-day precisions were 2.4% and 2.3% (n=7) for the elution peak height and elution peak area of LDL, respectively.Results reveal that AF4-UV/Vis is a useful tool for the separation and size characterization of LDL in egg yolk plasma.关键词
非对称场流分离/低密度脂蛋白/粒径表征/蛋黄浆质Key words
asymmetrical flow field-flow fractionation (AF4)/low density lipoprotein (LDL)/size characterization/egg yolk plasma分类
化学化工引用本文复制引用
张文惠,蔡春雪,王静,毛震,李月秋,丁良,申世刚,窦海洋..非对称场流分离技术在蛋黄浆质低密度脂蛋白粒径表征中的应用[J].色谱,2017,35(8):855-859,5.基金项目
河北省自然科学基金项目(B2016201002) （B2016201002）
河北省引进留学人员资助项目(CL201603) （CL201603）
河北省高等学校科技研究基金项目(QN2016081) （QN2016081）
河北省医学学科研究重点课题(20160048) （20160048）
保定市科学研究与发展计划项目(15ZG049).Natural Science Foundation of Hebei Province (No.B2016201002) （15ZG049）
Hebei Introduction Foundation for the Returned Overseas Scholars (No.CL201603) （No.CL201603）
Research Project of Science and Technique of Universities in Hebei Province (No.QN2016081) （No.QN2016081）
Important Project of Medicine Science and Research of Hebei Province (No.20160048) （No.20160048）
Project of Science Research and Development Plan of Baoding (No.15ZG049). （No.15ZG049）
