中国人兽共患病学报2017，Vol.33Issue(6)：473-480,8.DOI:10.3969/j.issn.1002-2694.2017.06.001
云南中缅边境登革1型病毒全基因组序列特征研究
Molecular characteristics of the full-length genome of dengue serotype 1 virus strains isolated from dengue fever cases in Sino-Myanmar border region in Yunnan Province, China
摘要
Abstract
We investigated the molecular characteristics of the full-length genome of 14 dengue serotype 1 virus (DENV-1)strains isolated in Sino-Myanmar border region in Yunnan Province,China during 2013-2015.Isolation of dengue virus was using C6/36 cell culture method.Viral RNA was extracted from virus isolates,and then the full-length genome was amplified by RT-PCR.The homology and phylogenetic analysis was made on the nucleotide and deduced amino acid sequences by bioinformatics software including ClastalX1.83 and MEGA6 etc.Results showed that fourteen strains of DENV-1 isolated from dengue fever cases,of these,9 strains from Ruili City of Dehong Prefecture,3 from Lincang Prefecture,2 from Kunming City.RT-PCR and sequencing indicated that the full-length genes (10 735 nt) of 14 DENV-1 strains were obtained,and their open reading frame (95-10 271) were coded 3 392 amino acid residues.The genotypes of DENV-1 were revealed by homology and phylogenetic analysis based on structural and non-structural proteins.Thirteen were genotype Ⅰ (G-Ⅰ) (7 from indigenous cases in Ruili and Lincang and 6 from imported case from Myanmar to Ruili,Lincang and Kunming),and 1 G-Ⅲ from imported case from India to Kunming.The phylogenic analysis indicated that the 13 isolates from Yunnan divided into 2 phylogenic subgroups,and they had a closer genetic relationship with the strains isolated from Southeast Asia.The gene sequences of the 13 G-Ⅰ strains have been acquired,the rate of their nucleotide homology and amino acid homology were 97.02 ％-100 ％ and 98.78 ％100 ％ respectively.Compared with 6 strains from Southeast Asia,nucleotide homology and amino acid homology were 96.53％-99.53％ and 97.33％-100％ respectively.Compared with prototype strain (US_Hawaii) of DENV-1,nucleotide homology and amino acid homology were 93.76％-94.45 ％ and 95.86 ％-96.91％ respectively.Compared with US_Hawaii strain,there were 44 and 150 different sites in amino acid of structural and non-structural proteins,respectively.The G-1 of DENV-1 have been popular in Sino-Myanmar border region in Yunnan,2013-2015.They have genetic diversity but multiple transmission sources were from Myanmar,and should strengthen control cross-border spread of dengue fever in this region.It is necessary to further study that change of the amino acid sites of Yunnan strains of DENV-1 is related to its antigenicity and pathogenicity.关键词
登革1型病毒/全基因组/系统进化分析/同源性分析/氨基酸位点分析Key words
dengue serotype 1 virus/full-length genome/phylogenetic analysis/homology analysis/amino acid site analysis分类
医药卫生引用本文复制引用
胡挺松,范泉水,张海林,刘永华,徐松淼,李华昌,邓波,尹小雄,黄瑛,张富强..云南中缅边境登革1型病毒全基因组序列特征研究[J].中国人兽共患病学报,2017,33(6):473-480,8.基金项目
国家十三、五重大专项(No.2016YFC1200100),全军青年培育项目(15QNP035)和中国博士后基金(No.2015M582907、2016T91009)联合资助 Supported by the National Key Basic Research Program of China (No.2016YFC1200100),the Science and Technology Programs of Military (No.15QNP035),and the General Financial Grant from the China Postdoctoral Science Foundation (Nos.2015M582907 and 2016T91009) （No.2016YFC1200100）
