中国人兽共患病学报2017，Vol.33Issue(6)：486-490,5.DOI:10.3969/j.issn.1002-2694.2017.06.003
白念珠菌CDR1/CDR2和MDR1基因高表达对氧化应激的影响
Effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans
摘要
Abstract
To investigate the effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans,we evaluated the effect of H2O2 on cell viability in C.albicans overexpressing genes CDR1 /CDR2 or MDR1 and their parent strains.After establishing an oxidative stress model with H2O2,we detected reactive oxygen species (ROS),mitochondrial membrane potential (Δψm) and the expression of oxidative stress response-related genes (CAP1 and GRP2) and ROS clearance related-genes (SOD2 and SOD5).The results showed that C.albicans growth were inhibited by 100％ after the treatment of 5 mmol/L H2O2.HeO2 caused more ROS accumulation and Δψm reduction in parent strains than in CDR1/CDR2 or MDR1 genes overexpressed strains (P＜0.05).Compared to parent strains,the up-regulated expression of CAP1 and GRP2 were relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains,moreover,the down-regulated expression of SOD2 and SOD5 were also relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains (P＜0.05).In conclusion,the overexpression of CDR1/CDR2 and MDR1 genes could reduce the oxidative stress response and enhance the adaptability of C.albicans to oxidative stress.关键词
白念珠菌/CDR1/CDR2基因/MDR1基因/高表达/氧化应激Key words
Candida albicans/CDR1/CDR2 gene/MDR1 gene/overexpression/oxidative stress分类
医药卫生引用本文复制引用
蒋玲,赵亚婧,李水秀,宋延君,郭慧,朱坤举,张宏..白念珠菌CDR1/CDR2和MDR1基因高表达对氧化应激的影响[J].中国人兽共患病学报,2017,33(6):486-490,5.基金项目
国家自然科学基金(No.81171542/81471995) Supported by the National Natural Science Foundation of China (No.81171542 / 81471995) （No.81171542/81471995）
