中国人兽共患病学报2017，Vol.33Issue(6)：491-494,4.DOI:10.3969/j.issn.1002-2694.2017.06.004
华支睾吸虫成虫抗原和排泄分泌产物对T细胞的作用研究
Effects of crude antigen and excretory-secretory products of Clonorchis sinensis on T cells
摘要
Abstract
We investigated the roles of the crude antigen(CA) of Clonorchis sinensis and excretory secretory products (ESPs) in the polarization of Th1 and Th2 cells.Bone marrow-derived cells were generated from BALB/c mice and isolated into immature DCs;immature DCs were then treated with either CA (CA stimulated group),ESPs (ESPs stimulated group),LPS (positive control group) or PBS (negative control group) for 24 hours.Then the CD4+T cells were isolated from mouse spleen by using anti mouse-CD4 Microbeads,and further cocultured with stimulated DCs for another 72 hours.The purities of DCs and CD4+ T cells were evaluated by flow cytometry and the expressing levels of T-bet mRNA and GATA-3 mRNA were detected by real-time PCR.ELISA was used to detect the levels of IFN-γ and IL-4 cytokines in the supernatant.mRNA levels of T-bet and GATA-3 in the ESPs group were higher than those in PBS-stimulated group (P＜0.05).The concentrations of IFN-γ and IL-4 cytokines in the culture were increased in the ESPs group,compared with PBS stimulated group(P＜0.05).IFN-γ but not IL-4 was increased in CA group (P＜0.05).The results implied that CA might play a role in Th1 type immune response,and ESPs likely play roles in both Th1 and Th2 immune responses.关键词
华支睾吸虫/DC细胞/CD4+细胞/成虫抗原/排泄/分泌产物Key words
Clonorchis sinensis/DCs/CD4+T cells/crude antigen/excretory-secretory products分类
医药卫生引用本文复制引用
张波,张蓓蓓,程晓丹,华慧,于倩,颜超,汤仁仙,郑葵阳..华支睾吸虫成虫抗原和排泄分泌产物对T细胞的作用研究[J].中国人兽共患病学报,2017,33(6):491-494,4.基金项目
国家自然科学基金(No.81171590,81572019) （No.81171590,81572019）
家畜疫病病原生物学国家重点实验室开放课题(No.SKLVEB2013KFKT005) （No.SKLVEB2013KFKT005）
江苏高校优势学科建设工程资助项目(PAPD)联合资助 Funded by the National Natural Science Foundation of China(Grant Nos.81171590,81572019),the Chinese Academy of Agricultural Sciences (Grant No.SKLVEB2013KFKT005),a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). （PAPD）
