中国人兽共患病学报2017，Vol.33Issue(6)：508-512,5.DOI:10.3969/j.issn.1002-2694.2017.06.007
分子信标探针技术用于PCR检测诺卡氏菌SecA1基因
PCR detection of the Nocardia gene SecA1 using molecular beacon probe
摘要
Abstract
Molecular beacon probe was designed based on a specific DNA sequence of Nocardia to PCR detection of thisbacterium.The strains of Nocardia、Gordina and Rhodococcus were inoculated in Brain Heart Infusion Agar medium separately,then the growth condition was observed,DNA was extracted as a template;the molecular beacon probe was designed based on the partial secA 1 gene sequences of Nocardia strains,and the probe was added into the reaction system of real time fluorescence quantitative PCR (RT-PCR),and the fluorescence signal was tested at the end of PCR.Showed that the amplified secA1 gene of Nocardia could produce positive fluorescence signal in RT-PCR,but those of Gordonia and Rhodococcus with control groups showed negative results because of no fluorescence signal.In conclusion as a housekeeping gene,secA1 is an ideal target molecule to identify the actinomycetes strains on the species level in the systematic evolution research,and the technique of fluorescence molecular beacon probe is accurate,rapid and sensitive for detecting the Nocardia strains with secA1 gene.关键词
诺卡氏菌属/SecA1基因/分子信标探针/实时荧光定量PCRKey words
Nocardia/SecA1 gene/molecular beacon probe/RT-PCR分类
医药卫生引用本文复制引用
王颜颜,夏茂宁,明春艳,黄劲,刘涛华,周兵,康颖倩..分子信标探针技术用于PCR检测诺卡氏菌SecA1基因[J].中国人兽共患病学报,2017,33(6):508-512,5.基金项目
国家自然基金(No.31260029),贵州省国际科技合作项目(黔科合外G字[2014]7006),贵阳市人民政府-贵州医科大学科学技术联合基金“贵阳市科技局创新团队”项目(GY2015-18)和贵州省科技计划项目(黔科合[2010]3154号)联合资助 Supported by the National Natural Science Fund of China(No.31260029),the International Science and Technology Cooperation Project of Guizhou(No.[2014]7006),the Technology Innovation Team Project of Guiyang(No.GY2015-18),and the Science and Technology Plan Project of Guizhou(No.[2010]3154) （No.31260029）
