中国实用口腔科杂志2017，Vol.10Issue(7)：417-422,6.DOI:10.19538/j.kq.2017.07.007
纯钛超声工作尖与传统超声工作尖对钛表面形态及人牙周膜细胞黏附强度影响的比较研究
Evaluation of the surface alterations and the adhesion strength of human periodontal ligament cells on titanium surface treated by pure titanium and conventional ultrasonic scaler tips
摘要
Abstract
Objective To compare the effects of pure titanium and conventional steel ultrasonic scaler tips on titanium surface and evaluate the adhesion strength of human periodontal ligament cells (hPDLCs)on the titanium surface treated by the two different tips.Methods Totally 60 smooth titanium discs were used in this research.The discs were randomly divided into five groups,each of which contained 12 discs.Ti10 group:discs were scaled for 10s with pure titanium tip;Ti30 group:discs were scaled for 30s with pure titanium tip;Cv 10 group:discs were scaled for 10s with conventional steel tip;Cv30 group:discs were scaled for 30s with conventional steel tip.Control group:discs were not scaled.After the treatment according to the protocols,the morphology and roughness of the discs were evaluated by scanning electron microscope (SEM)and profilometer separately.In order to test the biocompatibility of the treated titanium discs,hPDLCs were co-cultured with those discs for 12 hours,and the cell toxicity of treated discs and the adhesion strength of hPDLCs on titanium discs was calculated with CCK-8 method.Results SEM results showed that Ti10 group had less morphological changes than Cv10 group and this change was highly consistent with the profilometer results which also showed that the roughness of titanium at Ti10 group was significantly lower than Cv10 group (P ＜ 0.05).However,if the scaling time increased to 30 seconds,there were no statistical difference among groups.There were no significant difference among groups for the adhesion strength of hPDLCs after being treated by different scaler tips and no toxicity was found.Conclusion Pure titanium ultrasonic scaler tips are better than conventional ultrasonic scaler tip with respect to the protection of titanium surface,although there were no significant differences in the promotion of cell adhesion.关键词
纯钛超声工作尖/传统超声工作尖/人牙周膜细胞/表面粗糙度/黏附强度Key words
pure titanium ultrasonic scaler tip/conventional ultrasonic scaler tip/human periodontal ligament cell, hPDLC/surface roughness/adhesion strength分类
医药卫生引用本文复制引用
魏挺力,李厚轩,刘娟,陈畅行,杜志斌,闫福华..纯钛超声工作尖与传统超声工作尖对钛表面形态及人牙周膜细胞黏附强度影响的比较研究[J].中国实用口腔科杂志,2017,10(7):417-422,6.基金项目
江苏省医学创新团队(领军人才)(CXTDB2017014) （领军人才）
江苏省双创计划引进人才[苏人才办(2014)27号] （2014）
江苏特聘医学专家资助项目[苏卫人(2014)49号] （2014）
