中国实用口腔科杂志2017，Vol.10Issue(7)：423-426,4.DOI:10.19538/j.kq.2017.07.008
牙乳头细胞对大鼠牙髓干细胞增殖的影响及机制研究
Effects of dental papillae cells on the proliferation of dental pulp stem cells and its mechanism
摘要
Abstract
Objective To investigate effects of dental papillae cells on the proliferation of dental pulp stem cells (DPSCs) and its mechanism.Methods DPSCs and dental papillae cells were cultured,the co-cuhured systems of DPSCs and dental papillae cells were established.The intracellular mineralization nodes were observed.The cell cycle of DPSCs was detected by flow cytometry.Finally,when the inhibitor of Notch,PHI,was administrated,the expression of Notch1,Jagged1 mRNA and protein was examined.Results The obvious mineralization nodules were observed in DPSCs after 14 d of co-culture.Flow cytometry results showed that stratified co-culture promoted the proliferation of DPSCs.RTPCR and western blot results showed that the expression of Notch 1,Jagged 1 mRNA and protein in the co-culture group was significantly increased compared with control group.Conclusion Stratified co-culture of DPSCs and dental papillae cells might promote the mineralization and proliferation by activating Notch signal pathway.关键词
牙乳头细胞/牙髓干细胞/细胞增殖Key words
dental papillae cell/dental pulp stem cell/cell proliferation分类
医药卫生引用本文复制引用
张忠提,李琳..牙乳头细胞对大鼠牙髓干细胞增殖的影响及机制研究[J].中国实用口腔科杂志,2017,10(7):423-426,4.基金项目
辽宁省自然科学基金(2015020558) （2015020558）
辽宁省临床能力建设项目(LNCCC-D17-2015) （LNCCC-D17-2015）
