中国现代医学杂志2017，Vol.27Issue(17)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.17.001
青藤碱对胶原诱导性关节炎大鼠滑膜血管新生的影响
Effect of Sinomenine on synovial angiogenesis in rats with collagen-induced arthritis
摘要
Abstract
Objective To observe the effect of Sinomenine on the expression of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP-9) in rats with type Ⅱ collagen-induced arthritis (CIA), and to explore its therapeutic roles and mechanism. Methods Totally 40 male SD rats were recruited, among which 10 were randomly selected as the normal control group. The CIA model was established in the rest 30 rats by subcutaneous injection of type Ⅱ collagen of bovine emulsion in the tail root and induction of incomplete Freund's adjuvant. On day 15 primary immunization rats were randomly divided into 3 groups, i.e. model group, Tripterygium Glycosides (TG) group (at a daily dose of 9.68 mg/kg body weight), and Sinomenine (SIN) group (at a daily dose of 100 mg/kg body weight), 10 in each group. The corresponding medication was given to the rats of each group by gastrogavage once daily for 28 days. An equal volume of sodium chloride injection was given to the rats in the normal control group and the model group by gastrogavage, once daily for 28 successive days. The swelling degree of the joints was measured. The rats were sacrificed after 28-day treatment. Plasma levels of VEGF and MMP-9 were measured with ELISA. The expression of HIF-1α in synovium was detected by immunohistochemistry. Results Compared with the model group, the swelling degree of the joints was significantly alleviated in the TG group and the SIN group (P<0.05), the HIF-1α expression obviously decreased in the synovium of the TG group and the SIN group (P<0.05), the plasma levels of VEGF and MMP-9 were lower in the TG group and the SIN group (P<0.05). Conclusions SIN could markedly alleviate the swelling degree of joints in the CIA model rats. Its therapeutic efficacy is equal to that of TG. Its mechanism might be by down-regulating the HIF-1α expression in joints and the plasma levels of VEGF and MMP-9, thereby reducing synovial neovascularization.关键词
青藤碱/Ⅱ型胶原诱导性关节炎/低氧诱导因子1α/基质金属蛋白9Key words
Sinomenine/type Ⅱ collagen-induced arthritis/hypoxia-inducible factor-1α/vascular endothelial growth factor/matrix metalloproteinase-9分类
医药卫生引用本文复制引用
石宇红,彭琼,任亚飞,许佳,李丽梅,李宝贞,莫汉有..青藤碱对胶原诱导性关节炎大鼠滑膜血管新生的影响[J].中国现代医学杂志,2017,27(17):1-6,6.基金项目
国家自然科学基金(No:81460257) （No:81460257）
广西中医药管理局课题(No:gzzc1238) （No:gzzc1238）
广西省桂林市攻关课题(No:20140120-1-2) （No:20140120-1-2）
