中国现代医学杂志2017，Vol.27Issue(17)：7-12,6.DOI:10.3969/j.issn.1005-8982.2017.17.002
地塞米松抑制胆管瘢痕成纤维细胞的体外研究
Inhibitory effect of Dexamethasone on rabbit bile duct scar fibroblasts in vitro
摘要
Abstract
Objective To investigate the effect of Dexamethasone (DEX) on expressions of collagen I, connective tissue growth factor (CTGF), and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) genes in rabbit bile duct scar model fibroblasts (MF) in vitro. Methods Rabbit bile duct scar model was established to culture MF. The MF were identified, and then treated by different concentrations of DEX (0, 0.01, 0.05 and 0.25 mg/ml). After respective incubation for 48 h, cell proliferation was investigated by CCK-8; relative mRNA expressions of collagen-I, CTGF and PTEN were assessed by qRT-PCR; relative protein expressions of collagen-I and PTEN were investigated by Western blot. Results DEX significantly inhibited cell proliferation, down-regulated both mRNA and protein expressions of collagen-I and CTGF, and significantly up-regulated both mRNA and protein expressions of PTEN(P < 0.05) in the MF in a dose-dependent manner. Conclusions DEX may have preventive and therapeutic effect on bile duct scar and benign biliary stricture. Effect of DEX in anti-bile duct scar and benign biliary stricture may be partly through regulating collagen-I, CTGF and PTEN expressions in MF.关键词
胆管瘢痕/成纤维细胞/抑制/体外/地塞米松Key words
bile duct scar/fibroblast/inhibitory effect/in vitro/Dexamethasone分类
医药卫生引用本文复制引用
李克跃,石承先,汤可立,刘振华,黎涛,张帅民,徐贤刚..地塞米松抑制胆管瘢痕成纤维细胞的体外研究[J].中国现代医学杂志,2017,27(17):7-12,6.基金项目
贵州省科技厅—贵州省人民医院联合基金[No:黔科合LH字(2016)7146] （2016）
