中国现代医学杂志2017，Vol.27Issue(17)：19-24,6.DOI:10.3969/j.issn.1005-8982.2017.17.004
MicroRNA-224靶向CNNM1抑制前列腺癌血管生成的实验研究
miR-224 inhibits angiogenesis of prostate cancer by targeting cyclin protein M1
摘要
Abstract
Objective To investigate the impacts of miR-224 on biochemical recurrence and angiogenesis of prostate cancer. Methods Bioinformatics analysis and luciferase reporter assay were performed to predict the gene that can be directly inhibited by miR-224. Taylor database was used for confirming the expressions of CNNM1 and miR-224, and their correlations with biochemical recurrence of prostate cancer. Finally, the impacts of CNNM1 and miR-224 on angiogenesis of prostate cancer were explored by PC3 cell in vitro and in vivo. Results CNNM1 was directly regulated by miR-224, and their expression levels were negatively correlated in prostate cancer tissue ( r=-0.378, P<0.05). miR-224 was negatively correlated to, while CNNM1 was positively correlated to biochemical recurrence of prostate cancer (P<0.05). The expressions of CNNM1 and CD31 decreased in the PC3 cells overexpressing miR-224 (P<0.05). However, CNNM1 enhanced the expression level of CD31 (P<0.05). The immunochemical staining of the transplantation tumor in the nude mice displayed that miR-224 overexpression could suppress angiogenesis in the prostate cancer tissue. Conclusions miR-224 suppresses angiogenesis and biochemical recurrence of prostate cancer by targeting CNNM1.关键词
前列腺癌/microRNA-224/细胞周期调节蛋白1/生化复发/血管生成Key words
prostate cancer/miR-224/CNNM1/biochemical recurrence/angiogenesis分类
医药卫生引用本文复制引用
黄亚强,黄红星,麦智鹏,黎卫,郑轶群,袁润强..MicroRNA-224靶向CNNM1抑制前列腺癌血管生成的实验研究[J].中国现代医学杂志,2017,27(17):19-24,6.基金项目
中国博士后科学基金(No:2016M590842) （No:2016M590842）
广东省医学科研基金(No:A2016052) （No:A2016052）
广东省中山市科技计划(No:2016B1028) （No:2016B1028）
