中国现代医学杂志2017，Vol.27Issue(17)：25-28,4.DOI:10.3969/j.issn.1005-8982.2017.17.005
PTPN14对食管癌的抑制作用及其机制研究
PTPN14 inhibits proliferative ability of esophageal cancer cells via hippo/YAP pathway
摘要
Abstract
Objective To investigate the tumor-suppressing role of protein tyrosine phosphatase non-receptor type 14 (PTPN14) in esophageal carcinoma and the mechanism. Methods EC9706 cells were transfected with PTPN14 over-expression vector, and then PTPN14 expression in the EC9706 cells was detected by qRT-QPCR and Western blot. The proliferation of the EC9706 cells was analyzed by MTT. The cell cycle of the EC9706 cells was analyzed by flow cytometry. The expression of YAP was detected by Western blot. Results The expression level of PTPN14 was significantly upregulated in the EC9706 cells transfected with PTPN14 over-expression vector. After transfection, the proliferation of EC9706 cells was inhibited, the cell cycle was blocked at G1, and YAP expression was decreased. Conclusions PTPN14 may block cell cycle and inhibit proliferation of esophageal cancer cells by down-regulation of YAP.关键词
蛋白酪氨酸磷酸酶14/YAP/增殖/食管癌Key words
protein tyrosine phosphatase non-receptor type 14/YAP/proliferation/esophageal cancer分类
医药卫生引用本文复制引用
刘华松,徐兰兰,张军,郭家龙,林称意,原野,曾敏,程栋梁..PTPN14对食管癌的抑制作用及其机制研究[J].中国现代医学杂志,2017,27(17):25-28,4.基金项目
湖北省教育厅项目(No:B2016497) （No:B2016497）
