中国药房2017，Vol.28Issue(22)：3044-3047,4.DOI:10.6039/j.issn.1001-0408.2017.22.06
左归丸对化疗性卵巢早衰小鼠卵巢组织中缝隙连接蛋白43表达的影响
Effect of Zuogui Pill on the Expression of Gap Junction Protein 43 in Ovarian Tissue of Mice with Chemo-therapy-induced Premature Ovarian Failure
摘要
Abstract
OBJECTIVE:To study the effect of Zuogui pill on the expression of gap junction protein 43(Cx43)in ovarian tis-sue of mice with premature ovarian failure(POF)induced by intraperitoneal injection of cyclophosphamide(CTX),and explore its mechanism in the treatment of chemotherapy-induced POF. METHODS:72 mice were randomly divided into blank group,model group,Estradiol valerate tablet group (positive control,0.00013 g/kg),Zuogui pill low-dose,medium-dose,high-dose groups (13.65,40.95,122.85 g/kg)by body mass,10 in each group. Except for blank group,other groups were reduce POF model by ip CTX,once a day,for 20 d. Meanwhile,the mice were intragastrically administrated related medicines,once a day,for 30 d. After 2 h of last administration,reverse transcription-polymerase chain reaction method and Western blot method were used to detect the Cx43 mRNA and protein expressions in ovarian tissue respectively,and immunohistochemistry method was adopted to detect the distribution of Cx43 protein in ovarian tissue. RESULTS:Compared with blank group,Cx43 mRNA and protein expressions in ovarian tissue of mice in model group were obviously weakened,and the distribution of Cx43 protein in follicle and granulocytes were obviously reduced(P<0.01). Compared with model group,Cx43 mRNA and protein expressions in ovarian tissue of mice in Estradiol valerate tablet group and Zuogui pill medium-dose,high-dose groups were obviously strengthened,and the distribution of Cx43 protein in follicle and granulocytes were obviously increased(P<0.01). CONCLUSIONS:Zuogui pill can increase the Cx43 mRNA and protein expressions in ovarian tissue of CTX-induced POF mice,increase the distribution of Cx43 in follicle and granu-locytes and gap junction function,which may be one of the treatment mechanism of POF.关键词
左归丸/卵巢早衰/卵巢/缝隙连接蛋白43/小鼠Key words
Zuogui pill/Premature ovarian failure/Ovarian/Gap junction protein 43/Mice分类
医药卫生引用本文复制引用
孙晓峰,阳松威,贺又舜,陈聪,龚力民,罗令..左归丸对化疗性卵巢早衰小鼠卵巢组织中缝隙连接蛋白43表达的影响[J].中国药房,2017,28(22):3044-3047,4.基金项目
国家自然科学基金资助项目(No.81303004) （No.81303004）
湖南省科技厅资助项目(No.2009RS3018) （No.2009RS3018）
湖南省教育厅资助项目(No.09C727) （No.09C727）
