中国药房2017，Vol.28Issue(22)：3048-3052,5.DOI:10.6039/j.issn.1001-0408.2017.22.07
参附注射液对慢性充血性心力衰竭大鼠心肌miRNA表达谱的影响
Effect of Shenfu Injection on the Expression Profile of Myocardial miRNA in Rats with Chronic Conges-tive Heart Failure
摘要
Abstract
OBJECTIVE:To study the effect of Shenfu injection(SFI)on the expression profile of myocardial miRNA in rats with chronic congestive heart failure (referring to heart failure). METHODS:40 rats were randomly divided into sham operation group (normal saline,ip),model group (normal saline,ip),valsartan group (positive control,10 mg/kg,ig) and SFI group (0.75 mL/kg,im),10 in each group. Except for sham operation group,another groups were reduced heart failure. After modeling, rats in other groups received related medicines,once a day. Affymetrix miRNA V4.0 chip technology was conducted to analyze the miRNA expression in myocardial tissue of rats with heart failure after administration for 28 d,and screen the miRNA on common differential expression in myocardial tissue of rats in each group. The miRNA associated with heart failure was analyzed by thresh-old of differential gene expression multiple value greater than or equal to 1.1. Gene Ontology (GO) analysis was used to analyze functional classification and biological signaling pathway of differentially expressed genes. RESULTS:There were totally 29 miR-Nas on common differential expression and 7 miRNAs associated with heart failure (rno-miR-30c-1-3p, rno-miR-125b-5p, rno-miR-133a-5p,rno-miR-199a-5p,rno-miR-221-3p,rno-miR-146a-5p and rno-miR-1-3p). SFI can significantly downregulate the expressions of rno-miR-125b-5p,rno-miR-133a-5p,rno-miR-221-3p,rno-miR-1-3p in myocardial tissue of rats (P<0.05 or P<0.01). Results of GO analysis showed,miRNAs on differential expression were mainly related to signal transduction,cytoplasm and nucleotide binding. CONCLUSIONS:SFI plays the role of anti-heart failure by participating in the downregulation of miRNAs associated with heart failure process and then affecting related signal pathways transduction after the combination of cyto-plasm and nucleotide.关键词
参附注射液/慢性充血性心力衰竭/微小RNA/差异筛选/基因本体论分析Key words
Shenfu injection/Chronic congestive heart failure/miRNA/Differential screening/Gene ontology analy-sis分类
医药卫生引用本文复制引用
施洋,樊官伟..参附注射液对慢性充血性心力衰竭大鼠心肌miRNA表达谱的影响[J].中国药房,2017,28(22):3048-3052,5.基金项目
国家重点基础研究发展计划(973计划)课题(No.2012CB518404) （973计划）
教育部"创新团队发展计划"课题(No.IRT1276) （No.IRT1276）
