中国药房2017，Vol.28Issue(22)：3052-3056,5.DOI:10.6039/j.issn.1001-0408.2017.22.08
渐尖毛蕨二氢黄酮苷对糖尿病肾病大鼠肾上皮-间质转化的影响
Effect of Cyslosorus acuminatus Flavonone Glycoside on Kidney Epithelial-mesenchymal Transition in Rats with Diabetic Kidney Disease
摘要
Abstract
OBJECTIVE:To investigate the effect of Cyslosorus acuminatus flavonone glycoside (CAF) on kidney epitheli-al-mesenchymal transition(EMT)in rats with diabetic kidney disease(DKD). METHODS:Rats were randomly divided into nor-mal group(normal saline),model group(normal saline),positive group [rosiglitazone,0.4 mg/(kg·d)],CAF high-dose and low-dose groups [12.5,25 mg/(kg·d)],10 in each group. Except for normal group,other groups were intraperitoneally injected strepto-zotocin(60 mg/kg)+high fat diet to induce DKD,and intragastrically administrated related medicines in 13-16 weeks. After the ex-perimental period,fasting blood glucose level and serum creatinine(Scr),blood urea nitrogen(BUN)contents of rats were detect-ed,collagen deposition and basement membrane thickening in kidney tissue were observed. Immunohistochemistry was used to de-tect α-smooth muscle actin(α-SMA),fibronectin,epithelial cadherin(E-cadherin)expressions in kidney tissue,and Western blot was used to determine the glycogen synthase kinase 3β(GSK-3β),phosphorylated GSK-3β(p-GSK-3β),β-catenin expressions in kidney tissue. RESULTS:Compared with normal group,fasting blood glucose level,Scr and BUN contents in model group were significantly increased (P<0.01);kidney tissue showed obvious collagen deposition and basement membrane thickening;theα-SMA,fibronectin,β-catenin expression levels and GSK-3β phosphorylation degree in kidney tissue were significantly increased (P<0.01),while E-cadherin expression levels was significantly decreased(P<0.01). Compared with model group,fasting blood glucose level,Scr and BUN contents in each administration group were significantly reduced(P<0.05 or P<0.01);collagen depo-sition and basement membrane thickening in kidney tissue were significantly improved;the α-SMA,fibronectin,and β-catenin ex-pression levels and GSK-3β phosphorylation degree in kidney tissue were significantly decreased (P<0.05 or P<0.01),while E-cadherin expression levels in positive group and CAF high-dose group were significantly increased(P<0.01). CONCLUSIONS:CAF can inhibit the kidney EMT of rats with DKD,the molecular mechanism may be associated with downregulating β-catenin ex-pression and inhibiting GSK-3βphosphorylation inactivation.关键词
渐尖毛蕨/二氢黄酮苷/糖尿病肾病/上皮-间质转化/上皮细胞钙黏蛋白/糖原合成酶激酶3β/大鼠Key words
Cyslosorus acuminatus/Flavonone glycoside/Diabetic kidney disease/Epithelial-mesenchymal transition/Epitheli-al cadherin/Glycogen synthase kinase 3β/Rats分类
医药卫生引用本文复制引用
熊佳俊,陈镜楼,宋红萍..渐尖毛蕨二氢黄酮苷对糖尿病肾病大鼠肾上皮-间质转化的影响[J].中国药房,2017,28(22):3052-3056,5.基金项目
湖北省卫生计生科研基金(No.WJ2017M189) （No.WJ2017M189）
武汉市科学技术计划项目(No.2015071704011629) （No.2015071704011629）
