中国药房2017，Vol.28Issue(22)：3065-3068,4.DOI:10.6039/j.issn.1001-0408.2017.22.11
茯苓皮水提物对四氯化碳诱导大鼠肝纤维化的改善作用
Improvement Effect of Poria cocos Peels Water Extract on Liver Fibrosis in Rats Induced by Carbon Tetra-chloride
摘要
Abstract
OBJECTIVE:To study the improvement effect of Poria cocos peels water extract(PWE)on liver fibrosis in rats in-duced by carbon tetrachloride(CCl4). METHODS:84 rats were randomly divided into blank control group,solvent control group, model control group, positive control group (Compound biejia ruangan tablet, 0.75 g/kg), PWE low-dose, medium-dose, high-dose groups (0.9,1.8,3.6 g/kg,calculated by crude drugs),12 in each group. Except for blank control group and solvent control group(ip vegetable oil),other groups received CCl4-vegetable oil solution to reduce liver fibrosis model,ip. After model-ing,each administration group received related medicines,ig,other 3 groups received equal volume of normal saline,once a day, for 4 weeks. After administration,enzyme-linked immunosorbent assay was used to detect the aspartate aminotransferase(AST),al-anine aminotransferase(ALT),laminin(LN),hyaluronic acid(HA),hydroxyproline(Hyp)contents in serum and reduced gluta-thione (GSH),superoxide dismutase (SOD),malondialdehyde (MDA) contents in liver tissue of rats;HE staining and Masson staining were adopted to observe the pathological changes of liver tissue. RESULTS:Compared with blank control group,indexes of rats in solvent control group had no obvious changes(P>0.05). AST,ALT,LN,HA,Hyp contents in serum and MDA con-tent in liver tissue in model control group were significantly increased(P<0.05);GSH,SOD contents in liver tissue were signifi-cantly reduced(P<0.05);and liver tissue showed obvious fibrosis lesions. Compared with model control group,AST,ALT,LN, HA,Hyp contents in serum and MDA content in liver tissue in PWE medium-dose,high-dose groups were significantly reduced (P<0.05);GSH,SOD contents in liver tissue were significantly increased(P<0.05);fibrosis degree of liver tissue was obvious-ly relieved. CONCLUSIONS:PWE shows good improvement effect on liver fibrosis of rats induced by CCl4,which may be related to inhibiting the lipid peroxidation.关键词
茯苓皮/水提物/肝纤维化/脂质过氧化/大鼠Key words
Poria cocos peels/Water extract/Liver fibrosis/Lipid peroxidation/Rats分类
医药卫生引用本文复制引用
蒋征奎,王学方..茯苓皮水提物对四氯化碳诱导大鼠肝纤维化的改善作用[J].中国药房,2017,28(22):3065-3068,4.基金项目
河南省科技攻关项目(No.152102110115) （No.152102110115）
