中山大学学报（医学科学版）2017，Vol.38Issue(4)：489-493,5.
靶向乙型肝炎病毒的外源性microRNA的构建及作用
Construction and Effect of Artificial MicroRNA Targeted on Hepatitis B Virus
摘要
Abstract
[Objective] To investigate the construction and inhibitory effects of artificial microRNA targeted on hepatitis B virus.[Methods] Four artificial microRNA sequences for HBV gene were designed with Invitrogen online design software (BLOCK-iTTM RNAi Designer),amiRNA eukaryotic expression plasmid were constructed and transfected into human hepatocellular carcinoma cell line HepG2.2.15.Hepatitis B virus DNA in supernatant was detected by quantitative fluorescence PCR.HBsAg and HBeAg detected by ELISA.[Results] All of the four artificial microRNA sequences could down regulate the level of HBV-DNA,HBsAg and HBeAg.miR3 had the most significant effect on inhibiting HBV-DNA replication.[Conclusion] Artificial microRNA sequences can effectively inhibit the replication of HBV in vitro.关键词
microRNA/乙肝病毒/复制/RNA干扰Key words
nicroRNA/hepatitis B virus/replication/RNA interference分类
医药卫生引用本文复制引用
迟作华,刘爱梅,孙艳..靶向乙型肝炎病毒的外源性microRNA的构建及作用[J].中山大学学报（医学科学版）,2017,38(4):489-493,5.基金项目
国家自然科学基金(81000923) （81000923）
广东省科技计划项目(2013B021800089,2016A02021602,2014A020212468) （2013B021800089,2016A02021602,2014A020212468）
