中山大学学报（医学科学版）2017，Vol.38Issue(4)：505-511,7.
内质网应激参与蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝的形成
Involvement Endoplasmic Reticulum Stress on Process of Nonalcoholic Fatty Liver Disease Induced by MCD
摘要
Abstract
[Objective] To investigate the effect of the ER stress on the process of nonalcoholic fatty liver disease which is induced by methionine-choline deficient diet.[Methods] C57/BL mice were randomly divided into 8 groups (n =10),group 1-4was given normal diet,5-8 group was given MCD diet.Then took one group from both normal diet and MCD diet in the 2nd,4th,6th,8th week,recorded each group's body weight and liver weight.The liver fibrosis and inflammation in mice were analyzed by histopathological staining and NAS.The expressions of endoplasmic reticulum stress markers such as p-PERK,p-eIF2α,CHOP were detected by Western Blot and real time fluorescent quantitative PCR (q-PCR).[Results] ①The ALT and AST in serum,TG and TC in liver tissue were both higher in MCD group,the same as NAS;②The expressions of PERK,eIF2α,CHOP were significantly higher than the nommal diet group,while the GADD34 expression was lower.What's more,as time went by,the difference was more obvious;3.There was a significant correlation between the ERS index and NAS score in MCD group.[Conclusion]The liver damage and inflammatory response was lifted gradually in the mice fed with MCD diet.The ER stress may take an important role in this process.关键词
非酒精性脂肪肝/非酒精性脂肪肝炎活性评分/内质网应激Key words
nonalcoholic fatty liver disease/nonalcoholic steatohepatitis score/endoplasmic reticulum stress分类
医药卫生引用本文复制引用
方婉军,陈旭,凌文华..内质网应激参与蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝的形成[J].中山大学学报（医学科学版）,2017,38(4):505-511,7.基金项目
广东省科技计划项目(2013B09060013) （2013B09060013）
