中山大学学报（医学科学版）2017，Vol.38Issue(4)：526-531,6.
SGBG/PHBV与人牙周膜细胞的生物相容性
Biocompatibility between SGBG/PHBV and Human PDLC
摘要
Abstract
[Objective] The biocompatibility between SGBG/PHBV and human PDLC were investigated to provide a basis for the choice of the scaff material of periodontal tissue engineering.[Methods] Human PDLC were cultured using tissue-explant technique.Seeding on 96 wells plate,9 wells for one group,Four different concentrations (100％,75％,50％,25％,0％) of maceration extract of SGBG/PHBV were added into the culture plantsafter 48-h cell seeding,the grades of the cytotoxicity of SGBG/PHBV was evaluated by MTT assay.Human PDLC cultured in vitro were collected and seeded on the three-dimensional scaffolds of SGBG/PH-BV,the cellular morphology and cell growth on the scaffolds were observed and photographed by scanning electronic microscope.HumanPDLC seeded on the three-dimensional scaffolds of SGBG/PHBV in the experimental group,and human PDLC seeded by DMEM in the control group,after 12-,24-,and 48-h cell seeding,got 27 simples for each group,and the affection of the SGBG/PHBV on cell secretory function was observed by spectrophotometry which assayed the biochemical indexes ALP in supinate.[Results] The grades of the cytotoxicity of SGBG/PHBV were 0 and 1.It was displayed that human PDLC adhered and proliferated well on the scaffold of SGBG/PHBV under the scanning electronic microscope.The significant difference of ALP in supinate between the experimental group and the control group (P ＜ 0.05).[Conclusions] SGBG/PHBV had no cytotoxicity to human PDLC.SGBG/PHBV is potential to further study as the scaffolds of periodontal tissue engineering since it displayed the satisfactory biocompatibility with human PDLC.关键词
溶胶-凝胶生物活性玻璃/聚羟基丁酸戊酸共聚酯/人牙周韧带细胞/牙周组织工程Key words
SGBG/PHBV/PDLC/periodontal tissue engineering分类
医药卫生引用本文复制引用
黄南楠,孙文娟,吴坚,李婵秀,唐倩..SGBG/PHBV与人牙周膜细胞的生物相容性[J].中山大学学报（医学科学版）,2017,38(4):526-531,6.基金项目
广东省科技计划项目(2010B031100021) （2010B031100021）
