安徽中医药大学学报2017，Vol.36Issue(4)：79-82,4.DOI:10.3969/j.issn.2095-7246.2017.04.023
反向高效液相色谱法测定覆盆子中3种三萜酸的含量
Determination of Three Triterpene Acids in Rubi Fructus by Reverse-phase High-performance Liquid Chromatography
摘要
Abstract
Objective To establish a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of three triterpenes (maslinic acid, corosolic acid, and oleanolic acid) in Rubi Fructus, and to provide a basis for the quality assessment of Rubi Fructus.Methods RP-HPLC was performed on a WondaSil C18-WR column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile (A)-0.2% phosphoric acid solution (B) (9∶1) for isocratic elution at a column temperature of 25 ℃, a flow rate of 0.6 mL/min, a detection wavelength of 205 nm, and a sample size of 10 μL.Results The concentrations of maslinic acid, corosolic acid, and oleanolic acid showed a good linear relationship with the peak area within the ranges of 0.156-3.900 μg, 0.102-2.55 μg, and 2.04-51 μg, respectively, with average recovery rates of 95.00%, 91.67%, and 93.80%, respectively (relative standard deviations=2.59%, 3.25%, and 2.86%, respectively).Conclusion This method is simple and feasible and can be used for the determination of triterpenes in Rubi Fructus.关键词
覆盆子/山楂酸/科罗索酸/齐墩果酸/反相高效液相色谱法Key words
Rubi Fructus/Maslinic acid/Corosolic acid/Oleanolic acid/Reverse-phase high-performance liquid chromatography分类
医药卫生引用本文复制引用
曹富,邱晓霞,张玲,王元媛..反向高效液相色谱法测定覆盆子中3种三萜酸的含量[J].安徽中医药大学学报,2017,36(4):79-82,4.基金项目
安徽省高校自然科学研究重点项目(KJ2015A128) （KJ2015A128）
