北京生物医学工程2017，Vol.36Issue(4)：433-437,5.DOI:10.3969/j.issn.1002-3208.2017.04.018
三维细胞培养中支架的研究现状
Research advance in scaffords of three-dimensional cell culture
李彤 1张晓晓 1李婧 1王俊妹 1吴哲1
作者信息
摘要
Abstract
Cell culture technology is one of the most core and basic technologies in biotechnology.At present,the limitation and shortcomings of two-dimensional cell culture methods such as multi-well plates,flasks and Petri dishes are obvious.Three-dimensional cell culture can improve the local microenvironment,cell-cell and cell-extracellular matrix interactions,mimic the physiological conditions in vivo to enhance the cell function.Three-dimensional cell culture might be widely used in oncology,drug discovery and other fields in the future.Scaffold is one of the most popular methods for three-dimensional cell culture,which is equivalent to artificial extracellular matrix to mimic the complex three-dimensional structure and the main characteristics of living tissues.This review summarizes three-dimensional cell culture from two aspects:scaffold-based and scaffold-free three-dimensional cell culture,especially introduces the scaffolds.关键词
3D细胞培养/生理微环境/支架/细胞外基质Key words
three-dimensional cell culture/physiological microenvironment/scaffold/extracellular matrix分类
医药卫生引用本文复制引用
李彤,张晓晓,李婧,王俊妹,吴哲..三维细胞培养中支架的研究现状[J].北京生物医学工程,2017,36(4):433-437,5.
