草业学报2017，Vol.26Issue(7)：71-81,11.DOI:10.11686/cyxb2016374
利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性
Genetic diversity analysis of Kentucky Bluegrass cultivars by RAPDs
摘要
Abstract
There are many commercial cultivars and wild germplasms of Kentucky bluegrass (Poa pratensis),an important cool-season turfgrass widely cultivated in temperate regions worldwide.In this study,72 Kentucky bluegrass materials from China and elsewhere were collected and evaluated at the seedling stage by recording plant height,number of tillers,leaf width,and leaf color.All these traits differed significantly among the germplasms.Polymorphic bands were amplified from these germplasms by using random amplified polymorphic DNA (RAPD) primers.A total of 115 polymorphic bands (91％) was generated using 20 RAPD primers.The dendrogram grouped these germplasms into two main clusters.The first cluster contained four domestic varieties (‘KBG04’,‘KBG03’,‘Huhe 2’,and ‘Zahe’),while the second cluster contained the other varieties.The second group had a higher genetic similarity coefficient,and was divided into three subgroups:Group Ⅰ consisting mainly of imported varieties,and Group Ⅱ and Group Ⅲ with mainly wild germplasms.The clustering analysis based on the RAPDs data was partly consistent with the source of the materials,but there was no correlation with seedling phenotypic traits.关键词
草地早熟禾/RAPD/遗传多样性/聚类分析Key words
Kentucky bluegrass/RAPD (random amplified polymorphic DNA)/genetic diversity/clustering analysis引用本文复制引用
涂明月,李杰,何亚丽,李醒,李俊,袁晓君..利用RAPD标记鉴定草地早熟禾种质资源的遗传多样性[J].草业学报,2017,26(7):71-81,11.基金项目
国家自然科学基金青年科学基金项目(31502002)和沪农科攻字(2013)第1-1号资助. （31502002）
