草业学报2017，Vol.26Issue(7)：82-89,8.DOI:10.11686/cyxb2016358
饲粮添加苜蓿黄酮对奶牛瘤胃菌群的影响
Effects of alfalfa flavonoids as dietary additives on bacterial flora in the rumen of dairy cows
摘要
Abstract
The objective of this study was to examine the effects of alfalfa flavonoids on the bacterial flora in the rumen of dairy cows.Four primiparous Holstein cows fitted with ruminal cannulas were used in a 4 × 4 Latin square design and were fed a total mixed ration containing 0,20,60,or 100 g alfalfa flavonoids per day (group A-D,respectively).The experiment had four periods and each period lasted 24 days.The ruminal fluid was collected during each period.Total bacterial DNA was extracted from the ruminal fluid,and 16S RNA sequences were obtained using the Illumina MiSeq platform.In total,52747 operational taxonomic units (OTUs) were generated,and 16142 OTUs were shared by four groups,accounting for 30.60％ of the total OTUs.The largest amount of OTUs was in group A.As the amount of alfalfa flavonoids in the diet increased,the Shannon index first increased and then decreased,but the abundance-based coverage estimator and Chao 1 indexes were unaffected.Simpson's index was significantly higher for group C than for groups A and D (P＜0.05).There were more bacterial genera in group C than in groups B and A,but the number of phyla in each group was not affected by the amount of alfalfa flavonoids in the diet.At the phylum level,the relative abundance of SR1 tended to increase linearly (0.05＜P＜0.10),and the relative abundance of Synergistetes first decreased and then increased (0.05＜P＜0.10) as the amount of alfalfa flavonoids in the diet increased.At the genus level,the relative abundance of RC9 and SP3-e08 was higher in group B than in group A,while the relative abundance of Oribacterium was higher in group A than in group B.The relative abundance of Shuttleworthia increased linearly with increasing alfalfa flavonoids (P＜0.05),and the relative abundance of Shuttleworthia was significantly higher in group D than in group A.In a clustering analysis,the bacterial community structure was similar in groups A and B,and in groups C and D.In conclusion,dietary supplementation with alfalfa flavonoids can change the composition and community structure of ruminal bacteria,thus affecting metabolism and nutrient digestion.关键词
苜蓿/黄酮类化合物/微生物/瘤胃/奶牛Key words
alfalfa/flavonoids/microorganism/rumen/dairy cow引用本文复制引用
占今舜,邬彩霞,刘明美,苏效双,詹康,赵国琦..饲粮添加苜蓿黄酮对奶牛瘤胃菌群的影响[J].草业学报,2017,26(7):82-89,8.基金项目
江苏省高校优势学科建设工程项目(PAPD),江苏省农业三新工程项目(SXGC[2016]326)和江苏省高校研究生科研创新计划项目(KYZZ_0367)资助. （PAPD）
