草业学报2017，Vol.26Issue(7)：190-200,11.DOI:10.11686/cyxb2016430
CRISPR/Cas9系统:基因组定点编辑技术及其在植物基因功能研究中的应用
CRISPR/Cas9: A gene targeting technology and its application in the study of plant genetic function
摘要
Abstract
CRISPR/Cas (clustered regularly interspaced short palindromic repeats) was derived from the adaptive immune system of bacteria and archaea to resist attacks from invasive viruses and exogenous DNA.Comparing zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALENs),the type Ⅱ CRISPR/Cas9 system mediated by RNA breaks new ground for gene editing due to its efficiency,low cost and ease of operation for the study of genetic function.This review summarizes recent research and progress of the structure,mechanisms and application of CRISPR/Cas9 for the study of genetic function and crop breeding.We also investigate the potential future development of CRISPR/Cas9.关键词
基因编辑/CRISPR/Cas9系统/基因功能/分子育种Key words
gene editing/CRISPR/Cas9 system/gene function/molecular breeding引用本文复制引用
包爱科,白天惠,赵天璇,苏家豪..CRISPR/Cas9系统:基因组定点编辑技术及其在植物基因功能研究中的应用[J].草业学报,2017,26(7):190-200,11.基金项目
国家自然科学基金(31670405,31372360),甘肃省科技支撑计划项目(144FKCA058)和兰州大学中央高校基本科研业务费专项资金(lzujbky-2016-4)资助. （31670405,31372360）
