重庆医学2017，Vol.46Issue(22)：3028-3032,5.DOI:10.3969/j.issn.1671-8348.2017.22.002
酪酸梭菌对食物过敏小鼠肠道屏障功能的影响
Effects of clostridium butyricum on intestinal barrier function in food allergic mice
摘要
Abstract
Objective To investigate that butyrate-producing probiotic clostridium butyricum improves the intestinal epithelial barrier function in food allergic mice by regulating TWIK-related potassium channel-1 (Trek1) expression in intestinal epithelial cells.Methods An intestinal allergy mouse model was created,then the model construction effect was verified by detecting the related indicators by ELISA,flow cytometer.The change of small intestinal tissue permeability was detected by the Ussing chambers.The Trek1 expressions in mouse jejunum tissue in control group and allergy group were detected by Western blot and immunofluorescent method;in the Transwell system,T84 cells were used to establish epithelial cellular monolayer for exposing to the allergic mediators,the Trek1 mRNA and protein expression were detected by qRT-PCR and Western blot.Then the allergic mice were grouped and treated by different methods including normal saline,SIT,SIT/SB,SB,SIT/CB,CB,SIT/CB/Spadin,the expression of mice intestinal Trek1,intestinal barrier function and allergic reaction indicators were detected.Results Compared with the control group,the small intestinal Trek1 protein and tissue expression level in the food allergic mice were significantly decreased,the intestinal mucosal permeability was significantly increased,the differences were statistically significant(P＜0.05);after T84 cells exposing to the allergic mediators,Trek1 mRNA and protein expression were significantly decreased(P＜0.05),but adding p38 inhibitor in advance could antagonize this change;the single use of SIT,clostridium butyricum and sodium butyrate could increase the intestinal Trek1 expression in food allergic mice,and alleviated the allergic reaction(P＜0.05),whereas the combined use of SIT and clostridium butyricum or sodium butyrate had more significant effect(compared with the SIT group,P＜0.05),moreover could significantly decrease small intestinal mucosal permeability and improved the intestinal barrier function(P＜0.05).Conclusion Sodium butyrate or butyrate-producing probiotic clostridium butyricum can restore the intestinal barrier function,alleviates the allergic reaction and strengthens the SIT curative effect in allergic mice by increasing Trek1 expression.关键词
食物过敏/Trek1/肠道屏障功能/益生菌Key words
food hypersensitivity/Trek1/epithelial barrier/probiotics分类
医药卫生引用本文复制引用
李旌,黄煌,梅璐,于泳,刘思濛,丁一芮,白利梅,蒋杰,郑鹏远..酪酸梭菌对食物过敏小鼠肠道屏障功能的影响[J].重庆医学,2017,46(22):3028-3032,5.基金项目
国家自然科学基金资助项目(81370494) （81370494）
国家973计划前期研究专项(2011CB512006) （2011CB512006）
河南省科技创新杰出人才基金(134200510022) （134200510022）
郑州市创新型科技人才队伍建设工程基金(131PLJRC656). （131PLJRC656）
