重庆医学2017，Vol.46Issue(22)：3036-3038,3042,4.DOI:10.3969/j.issn.1671-8348.2017.22.004
外源性心钠素对小鼠过敏性哮喘气道炎症的影响及其机制分析
Effect of exogenous atrial natriuretic peptide on airway inflammation in mice with allergic asthma and its mechanism
摘要
Abstract
Objective To investigate the effect of exogenous atrial natriuretic peptide on airway inflammation in allergic asthma mice model and its mechanism.Methods A total of 48 SPF 48 BALBc mice were selected and randomly divided into the normal group (equal quantity normal saline intervention),model group (equal quantity normal saline intervention),experimental group A (exogenous atrial natriuretic peptide 0.5 μg/g) and experimental group B(exogenous atrial natriuretic peptide 0.5 μg/g-+-A71915).The model group,experimental group A and B were given the corresponding treatment measures after successfully constructing the model,at 24 after the last provocation test,serum and bronchoalveolar lavage fluid in each group were collected for detecting inflammation factors.The HE staining was used to observe the pathological changes of lung tissue,the expression level of GATA3 protein in lung tissue was detected by Western blot technology.Results The blood EOS,lymphocyte and neutrophil ratio in the model group were higher than those in the normal group (P＜0.05),which in the experimental group A were higher than those in the model group and experimental group B (P＜0.05);bronchoalveolar lavage fluid EOS,lymphocytes,neutrophil ratio,IL-6,TNF-α levels in the model group were higher than those in the normal group (P＜0.05),alveolar lavage fluid EOS,lymphocyte,neutrophil count,IL-6 and TNF-α levels in the experimental group A were higher than those in the model group and experimental group B (P＜0.05);the expression level of GATA3 protein in lung tissue of the model group was higher than that in the normal group(P＜0.05),the expression level of lung tissue GATA3 protein in the experimental group A was higher than that in the model group and experimental group B (P＜0.05).Conclusion Exogenous atrial natriuretic peptide can further aggravate airway inflammation reaction in allergic asthma mice model,which is related to the activation of inflammatory cells,the release of inflammatory cytokines and the increase of GATA3 protein expression in lung tissue.关键词
心钠素/哮喘/小鼠/外源性/气道炎症/作用机制Key words
atrial natriuretic factor/asthma/mice/exogenous/airway inflammation/action mechanism分类
医药卫生引用本文复制引用
刘振宽,邢晓莉,李娜..外源性心钠素对小鼠过敏性哮喘气道炎症的影响及其机制分析[J].重庆医学,2017,46(22):3036-3038,3042,4.基金项目
天津市卫生局资助基金项目(tj20150823). （tj20150823）
