分析测试学报2017，Vol.36Issue(6)：800-804,5.DOI:10.3969/j.issn.1004-4957.2017.06.017
全自动免疫亲和在线净化/高效液相色谱法快速高通量测定饲料中黄曲霉毒素
Rapid and High Throughput Analysis of Aflatoxins in Feed Matrix Using On-line Immunoaffinity Column Clean-up and High Performance Liquid Chromatography
摘要
Abstract
A rapid analytical method using on-line immunoaffinity column clean-up and high performance liquid chromatography(HPLC) was developed for the determination of aflatoxins(B1,B2,G1,G2) in feed samples.The samples were extracted with acetonitrile-water solution (80 ∶ 20,by volumn).The extraction solution was diluted by 10 times using 3 g/L triton X-100 water solution,then injected into the RIDA(R) CREST on-line immunoaffinity column clean-up system,and washed out with methanol-water(45 ∶ 55,by volume) as mobile phase at a flow rate of 1.0 mL/min.Aflatoxins were analyzed by HPLC/FLD with post-column photochemical derivatizaton.The detection limits of AFB1,AFB2,AFG1 and AFG2 were 0.08,0.05,0.18,0.08 μg/kg,respectively.The linear ranges forAFB1,AFB2,AFG1 and AFG2 were 1-100,0.24-24,0.56-56,0.24-24μg/kg,with their correlation coefficients(r2) of 0.999 4,0.999 7,0.999 8 and 0.999 8,respectively.The recoveries for four kinds of feed samples spiked with aflatoxins were in the range of 72.6％-103％,with relative standard deviations of 2.5％-4.9％.Each immunoaffinity colomn could be used for 60 samples cleanup,and the total running time for each HPLC analysis is 15 min,thus 70-80 samples could be detected per day.This method could satisfy the demands for rapid and accurate analysis of aflatoxin in feed.关键词
饲料/黄曲霉毒素/在线免疫亲和柱净化/高效液相色谱Key words
feed/aflatoxins/on-line immunoaffinity column clean-up/high performance liquid chromatography (HPLC)分类
化学化工引用本文复制引用
李丽,黎睿,谢刚,叶金,王松雪..全自动免疫亲和在线净化/高效液相色谱法快速高通量测定饲料中黄曲霉毒素[J].分析测试学报,2017,36(6):800-804,5.基金项目
粮食公益性行业科研专项(201313005) （201313005）
