分析化学2017，Vol.45Issue(8)：1103-1108,6.DOI:10.11895/j.issn.0253-3820.170201
基于含有活性铜的碳点电化学传感器选择性检测尿酸
Determination of Uric Acid Based on Active Copper-Containing Carbon Nanodots-modified Electrochemical Biosensor
摘要
Abstract
The active copper-containing carbon nanodots were prepared by hydrothermal method, and then characterized by fluorescence spectroscopy and UV-visible absorption spectroscopy.Subsequently, a highly sensitive and selective electrochemical biosensor was fabricated on the basis of this synthesized carbon nanodots with electro-deposition technique.The electrode behavior was investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry.Furthermore, the catalysis mechanism was studied.The experimental results indicated that the biosensor exhibited a strong electrocatalytic activity toward the oxidation of uric acid (UA).What′s more, the interference from ascorbic acid and dopamine was eliminated effectively.Under the optimum conditions, there were linear relationships between the anodic peak current and the concentration of UA (1.00-300.0 μmol/L), and the limit detection was 0.30 μmol/L (S/N=3).The prepared biosensor had advantages such as easy fabrication, strong anti-interference ability, high sensitivity, and wide detection range, and could be used for real sample detection.关键词
活性铜/碳点/电化学/生物传感器/尿酸Key words
Active copper/Carbon nanodots/Electrochemistry/Biosensor/Uric acid引用本文复制引用
刘红英,金腾飞,戴大响,焦明儒,黄成,张名镇,顾春川..基于含有活性铜的碳点电化学传感器选择性检测尿酸[J].分析化学,2017,45(8):1103-1108,6.基金项目
本文系国家自然科学基金(No.21405029)、浙江省公益技术应用研究(分析测试)计划项目(No.2016C37042)、2016年度浙江省科协育才工程资助项目(No.2016YCGC007)、杭州市社会发展科研自主申报项目(No.20160533B70)、浙江省医药卫生科技计划(No.2017KY533)和杭州电子科技大学研究生科研创新基金(No.ZX160602317029)资助.This work was supported by the National Natural Science Foundation of China (No.21405029) （No.21405029）
