分析化学2017，Vol.45Issue(8)：1149-1154,6.DOI:10.11895/j.issn.0253-3820.170165
新型四肽亲水材料用于糖肽的高效富集
Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High-efficiency Glycopeptide Enrichment
摘要
Abstract
In this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed.The tetrapeptide modified silica gel materials (denoted as Poly-DAPD) were synthesized by atom-transfer radical-polymerization (ATRP) and characterized by N2 adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X-ray photoelectron spectrometer (XPS).The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel.Poly-DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid-phase extraction (SPE) mode.Comparing with commercialized ZIC-HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as-prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti-interference property.The SPE results demonstrated that the tetrapeptide-based HILIC materials could be a potential tool in large-scale glycosylation analysis.关键词
亲水作用色谱/四肽/糖肽富集/质谱Key words
Hydrophilic interaction chromatography/Tetrapeptide/Glycopeptide enrichment/Mass spectrometry引用本文复制引用
陈成,王宏喜,康虹健,姚要,卿光焱,李秀玲,梁鑫淼..新型四肽亲水材料用于糖肽的高效富集[J].分析化学,2017,45(8):1149-1154,6.基金项目
本文系国家自然科学基金项目 (No.21475129)资助 （No.21475129）
This work was supported by the National Natural Science Foundation of China (No.21475129) （No.21475129）
