干旱地区农业研究2017，Vol.35Issue(3)：190-197,8.DOI:10.7606/j.issn.1000-7601.2017.03.30
NO3--N/NH4+-N不同配比下紫花苜蓿氮代谢关键酶及钼、铁元素含量研究
Study on key enzyme activity in nitrogen metabolism and the content of molybdenum and iron in alfalfa under different NO3--N/NH4+-N ratio
摘要
Abstract
By solution culture method,‘Gannong No.3’ alfalfa was chosen as the experimental material to explore the effects of NO3--N/NH4+-N ratios(1/7、1/3、3/5、5/5、5/3、3/1、7/1) based on nitrogen supply of 210 rmg· L-1 on the activity of nitrate reductase(NR) and nitrogenase,and the content of molybdenum(Mo) and iron(Fe) in organs of alfafa during the whole growth period.The results showed that:(1) NR activity and Mo content was consistent:leaf ＞ root ＞ stem,while Fe content was root ＞ leaf ＞ stem.With the proportion of NO3--N increasing,NR activity in each organ became higher.In the early stage,when NO3--N/NH4 +-N equaled to 7/1,NR activity was the highest.The influence of different ratio on Mo content in root during seedling stage was similar to NR activity,that is to say,in large proportion of NO3--N it was the highest.During budding stage,the same as nitrogenase activity,in 1/7 it was highest.During mature stage,same as nitrogenase and NR activity,in 5/3 it was highest.The influence of different ratio on Fe content in root was similar to nitrogenase activity,that is to say,it was highest at 1/7 in seedling and 5/3 from fullbloom to mature.(2) NR and nitrogenase activity showed a single-peak curve during the whole growth period,that is to say,NR activity was the highest during full-bloom stage and nitrogenase activity was the highest during podding stage.NR activity was higher under larger proportion of NO3--N in different ratios during the whole growth period.Nitrogenase activity was the highest in 1/7 at seedling and budding,and from full-bloom to mature it was the highest in 5/3.Mo and Fe content in the stem and leaf was consistent,they were the highest for larger proportion of NH4+-N from budding to full-bloom and highest for larger proportion of NO3--N from podding to maturity.(3) Relationship between NR and nitrogenase activity differed in the whole growth period.At the early growth stage (from seeding to budding),NR activity was highest in 7/1 and lowest in 1/7.Nitrogenase activity was in the opposite situation,suggesting that there was an antagonistical relationship between NR and nitrogenase activity.From full-bloom to mature,both NR and nitrogenase activity was highest in 5/3,they accelerated each othcr.关键词
NO3--N/NH4+-N配比/紫花苜蓿/氮代谢/酶/钼/铁Key words
NO3--N/NH4 +-N ratio/alfalfa/nitrogen metabolism/enzyme/molybdenum/iron分类
自科综合引用本文复制引用
郝凤,刘晓静,范俊俊..NO3--N/NH4+-N不同配比下紫花苜蓿氮代谢关键酶及钼、铁元素含量研究[J].干旱地区农业研究,2017,35(3):190-197,8.基金项目
国家自然科学基金项目(31460622) （31460622）
公益性行业(农业)科研专项(201403048-8) （农业）
甘肃省科技支撑项目(1504NKCA003) （1504NKCA003）
