临床与病理杂志2017，Vol.37Issue(6)：1124-1129,6.DOI:10.3978/j.issn.2095-6959.2017.06.008
没食子酸通过活化线粒体通路诱导CNE-2鼻咽癌移植瘤细胞的凋亡
Gallic acid induced CNE-2 cell apoptosis on xenograft model in nude mice through mitochondria-dependent pathway
摘要
Abstract
Objective:To investigate the effects of gallic acid (GA) on the xenograft model of nasopharynx cancer in nude mice and to study its possible mechanism.Methods:Xenograft model of human nasopharynx cancer was established by transplantation of human nasopharynx cancer cell CNE-2.The mice were divided into 6 groups:the control group,the nasopharynx cancer group,the galllic acid (10 μg/kg) group,the galllic acid (20 μg/kg) group,the galllic acid (40 μg/kg) group and the DDP group.The tumor dimensions and volume were measured and the tumor inhibiting rate was calculated.The apoptotic rate of CNE-2 cells was examined with flow cytometry.The protein levels of cytochrome C,cleaved-cas 9,cleaved-cas 3 and cleaved-PARP were detected by Western blotting.Results:Compared with the nasopharynx cancer group,the tumor volume and weight significantly decreased in the galllic acid group,and the inhibitory rate and apoptotic rate of the tumor dose-dependently increased.Moreover,galllic acid enhanced the release ofcytochrome C from mitochondria to cytoplasm,leading to increase the protein levels of cleaved-cas 9,cleaved-cas 3 and deaved-PARP.Conclusion:Galllic acid induces apoptosis of nasopharynx cancer cell CNE-2 through mitochondria-dependent pathway.关键词
没食子酸/鼻咽癌瘤/抑瘤率/Caspase/线粒体通路Key words
gallic acid/nasopharyngeal tumor/tumor inhibiting rate/caspase/mitochondria-dependent pathway引用本文复制引用
吴艳林,蔡政,张明智,付晓瑞..没食子酸通过活化线粒体通路诱导CNE-2鼻咽癌移植瘤细胞的凋亡[J].临床与病理杂志,2017,37(6):1124-1129,6.基金项目
河南省教育厅科学技术研究重点资助项目(13A320413).This work was supported by the Science and Technology Research Key Project Foundation of Henan Province,China (13A320413). （13A320413）
