临床与病理杂志2017，Vol.37Issue(6)：1146-1152,7.DOI:10.3978/j.issn.2095-6959.2017.06.012
非小细胞肺癌患者ALK,EGFR及KRAS基因的检测及临床病理特征
Detection and clinicopathological features of ALK, EGFR and KRAS in non-small cell lung cancer patients
摘要
Abstract
Objective:To investigate the mutation frequency of anaplastic lymphoma kinase (ALK) fusion gene,epidermal growth factor receptor (EGFR) gene,and Kirsten rat sarcoma (KRAS) gene in Han patients with non-small cell lung cancer and their relationship with clinical pathological features.Methods:The ALK fusion gene abnormal expression was detected by IHC;the EGFR and KRAS gene mutation status were detected by PCR.The data were analyzed by X2 test and Fisher's exact probability method.Results:A total of 2 267 cases were examined for ALK fusion gene.Among the total sample population,1 655 cases were simultaneously examined for EGFR mutation,951 cases among 2 267 were tested by KRAS.The mutation frequency of ALK fusion gene,EGFR gene and KRAS gene were 7.28％ (165/2 267),48.58％ (804/1 655) and 11.40％ (108/947),respectively.ALK gene mutations occur more common in young,adenocarcinoma patients;EGFR gene mutations occur more common in women,adenocarcinoma patients;KRAS gene mutations occur more common in elderly,male,adenocarcinoma patients.There were 6 cases of double gene mutation in 1 655 cases of ALK and EGFR detection,a total of 4 double gene mutation cases were detected in 947 cases of ALK and KRAS simultaneously.No double mutation cases were detected in 943 cases of simultaneous EGFR and KRAS mutation detection.Conclusion:The mutations of ALK,EGFR and KRAS genes in patients with non-small cell lung cancer were associated with the age,sex and histological type of patients.Mutations in the ALK fusion gene may coexist with EGFR or KRAS mutations in individual cases.关键词
非小细胞肺癌/ALK融合基因/EGFR突变/KRAS突变Key words
non-small cell lung cancer/ALK fusion gene/EGFR mutation/KRAS mutation引用本文复制引用
刘静,姜桔红,顾莹莹,廖炫之,李谨,赵瑾,付琳,李龙光..非小细胞肺癌患者ALK,EGFR及KRAS基因的检测及临床病理特征[J].临床与病理杂志,2017,37(6):1146-1152,7.基金项目
广州市属高校科研项目(1201620051).This work was supported by Guangzhou Municipal Scientific Research Projects,China (1201620051). （1201620051）
