临床与病理杂志2017，Vol.37Issue(7)：1325-1330,6.DOI:10.3978/j.issn.2095-6959.2017.07.001
ITIH4基因沉默对人肝癌细胞HepG2增殖、迁移及侵袭的影响
Effect of ITIH4 gene silence on proliferation, migration and invasion of human hepatocellular carcinoma cell HepG2
摘要
Abstract
Objective: To investigate the effect of inter-alpha-trypsin inhibitor heavy chain H4 gene silence on proliferation, migration and invasion of human hepatocellular carcinoma cell HepG2.Methods: Four ITIH4 silencing vectors were delivered in to HepG2 cells by transfection. Stable cell clones were selected by 2 μg/mL puromycin. Silencing efficiency was evaluated by Western Blot and cell clone with the highest silencing efficacy (si-ITIH4) was used in the following study. Cell clone transfected with scramble sequence was used as normal control (si-Control). Cell counting and CCK-8 assay were applied to assess cell proliferation. Transwell assay was performed to evaluate cell migration and invasion.Results: We successfully obtained a HepG2 cell line with ITIH4 gene silencing named si-ITIH4. The silencing efficiency was 86% in compassion with si-Control. Compared to the si-Control group, si-ITIH4 cells showed significantly slower proliferation, decreased migration and mitigated invasion.Conclusion: Silence of ITIH4 significantly inhibited proliferation, migration and invasion of HepG2 cells. This study suggests that ITIH4 might be essentially involved in onset and progression of hepatocellular carcinoma.关键词
间-α-胰蛋白酶抑制剂重链H4/增殖/迁移/侵袭/肝细胞癌Key words
inter-alpha-trypsin inhibitor heavy chain H4/proliferation/migration/invasion/hepatocellular carcinoma引用本文复制引用
段煜,姜文文,高鹏,唐泽耀..ITIH4基因沉默对人肝癌细胞HepG2增殖、迁移及侵袭的影响[J].临床与病理杂志,2017,37(7):1325-1330,6.基金项目
辽宁省自然科学基金(201602206).This work was supported by Nature Science Foundation of Liaoning Province, China (201602206). （201602206）
