临床与病理杂志2017，Vol.37Issue(7)：1341-1347,7.DOI:10.3978/j.issn.2095-6959.2017.07.003
柴芪汤对非酒精性脂肪肝大鼠模型血清TNF-α和IL-6表达的影响
Effect of Chaiqi decoction on the level of TNF-α and IL-6 in mouse model with non-alcoholic fatty liver disease
摘要
Abstract
Objective: To observe the influence of Chaiqi decoction on the level of TNF-α and IL-6 in serum of mice model with NAFLD, and study the mechanism of dispersing stagnated liver and invigorating spleen therapy in preventing the progress of NAFLD.Methods: Forty SD mice were randomized into a normal group, a NAFLD model group and a Chaiqi decoction treat group. The mice, except the normal group, were fed with high fat for 8 weeks. Then 8 mice in the normal group and the NAFLD model group were executed respectively to evaluate the model of NAFLD. Afler the model made, the treat group accepted gastric lavage of Chaiqi decoction (5.67 g/kg) each day for 8 weeks. Then all mice were executed to detect the level of TG, TC, LDL-C, HDL-C, TNF-α and IL-6 in serum of mice with NAFLD, and the insulin resistance index (IRI) from fasting blood glucose (FBG) and fasting insulin (FINS). The pathology of liver were observed through microscope.Results: After fed with high fat diet for 8 weeks, the level of ALT, AST, FBG, FINS and IRI in serum of mice were higher than that in the normal group, and the diflerences were statistically signiflcant (P<0.05), fatty degeneration can be seen in liver cells. Afler threated with Chaiqi decoction for 8 weeks, the level of ALT, AST, TG, TC, LDL-C, TNF-α and IL-6 in serum were lower than that in the model group, the differences were statistically significant (P<0.05), fatty degeneration in liver cells were better than the mice in model group.Conclusions: Afler fed with high fat diet for 8 weeks, we succeed in building up NAFLD model in mice. Chaiqi decoction can reduce the level of inflammatory response and fatty degeneration in liver cells, lower down the expression of TNF-α and IL-6 in serum. It also can lower the level of TG, TC and LDL-C in in serum of mice with NAFLD and improve IR, too.关键词
非酒精性脂肪肝/柴芪汤/肿瘤坏死因子α/白介素6Key words
non-alcoholic fatty liver disease/Chaiqi decoction/TNF-α/IL-6引用本文复制引用
郭子宁,张立平,陈丽如,彭龙..柴芪汤对非酒精性脂肪肝大鼠模型血清TNF-α和IL-6表达的影响[J].临床与病理杂志,2017,37(7):1341-1347,7.基金项目
国家自然科学基金(81273695 （）
81503473).This work was supported by the National Natural Science Foundation of China (81273695 （）
81503473). （）
