临床与病理杂志2017，Vol.37Issue(7)：1356-1360,5.DOI:10.3978/j.issn.2095-6959.2017.07.005
黄连素对实验性脑出血大鼠脑水肿与神经功能的影响
Effect of berberine on cerebral edema and neurological function in intracerebral hemorrhage rats
关庆凯 1赵树鹏 1刁玉领 1周祥 1黄立勇 1金保哲 1周文科1
作者信息
- 1. 新乡医学院第一附属医院神经外科,河南 卫辉 453100
- 折叠
摘要
Abstract
Objective: To investigate the effects of Berberine on cerebral edema and neurological function in experimental intracerebral hemorrhage (ICH) rats and to explore the underlying mechanisms.Methods: Rats were randomly divided into flve groups: sham group, ICH group, ICH rats treated with low, medium or high dose of Berberine group. ICH model was induced by injection of collagenase, and ICH rats were received Berberine by gavage once a day at dose of 10, 20 or 40 mg/kg, respectively. Afler 28-day treatment, the grade of neurological function was scored, the water content of brain was measured, the apoptotic cells in pre-hematoma area were detected with TUNEL staining, and the protein expression of Cleaved cas-3, Cleaved cas-9 and the release of cytochrome c from mitochondria to cytoplasm were determined by Western blot.Results: Compared with the ICH group, neurological function improved significantly and the brain water content decreased, as accompanied with the reduced number of apoptotic cells in pre-hematoma area. Moreover, the protein expression of Cleaved cas-3, Cleaved cas-9 and the release of cytochrome c from mitochondria to cytoplasm were dramatically inhibited afler berberine treatment.Conclusion: Berberine protects against ICH-induced the impairment of neurological function through inhibition mitochondria-dependent intrinsic apoptosis pathway.关键词
黄连素/脑出血/线粒体凋亡通路Key words
berberine/intracerebral hemorrhage/mitochondria-dependent intrinsic apoptosis pathway引用本文复制引用
关庆凯,赵树鹏,刁玉领,周祥,黄立勇,金保哲,周文科..黄连素对实验性脑出血大鼠脑水肿与神经功能的影响[J].临床与病理杂志,2017,37(7):1356-1360,5.
