临床与病理杂志2017，Vol.37Issue(7)：1372-1375,4.DOI:10.3978/j.issn.2095-6959.2017.07.008
PTEN在人脑星形细胞瘤中的表达及意义
Expression of PTEN in human astrocytoma and its clinical significance
李全 1王广 2冯艳萍1
作者信息
- 1. 承德县中医院外一科,河北 承德 067400
- 2. 承德医学院附属医院神经外科,河北 承德 067000
- 折叠
摘要
Abstract
Objective: This study aimed to investigate the expression of phosphatase and tensin homology deleted on chromosome ten (PTEN) in human astrocytoma and its clinical signiflcance.Methods: SP immunohistochemical and RT-PCR were used to detect the expression of PTEN in normal brain and human astrocytoma. Western blot analysis were used to detect the expression of PTEN in cerebrospinal fluid (CSF) of human astrocytoma and normal brain.Results: Immunohistochemical assay showed that positive rates of PTEN in normal brain were 90% (9/10), which were higher than that in human astrocytoma 47.5% (19/40), and negatively correlated with histological diflerentiating degree. RT-PCR showed that, the relative expression levels of PTEN in normal brain were 0.861±0.072, which were higher than that in human astrocytoma (0.127±0.008), the diflerence was statistically significant (P<0.05). Western blot showed that the relative expression levels of PTEN in CSF of normal brain were 1.549±0.194, which were higher than that in human astrocytoma (0.602±0.058) (P<0.05). Conclusion: expression of PTEN can be helpful in the prediction of the biological behavior and prognosis of human astrocytoma.关键词
星形细胞瘤/磷酸酶-张力蛋白基因/Western印迹法/RT-PCR/免疫组织化学法Key words
human astrocytoma/phosphatase and tensin homology deleted on chromosome ten/Western blot/RT-PCR/SP immunohistochemistry引用本文复制引用
李全,王广,冯艳萍..PTEN在人脑星形细胞瘤中的表达及意义[J].临床与病理杂志,2017,37(7):1372-1375,4.
