神经损伤与功能重建2017，Vol.12Issue(4)：286-289,4.DOI:10.16780/j.cnki.sjssgncj.2017.04.002
可溶性环氧化物水解酶抑制剂TPPU对大鼠局灶脑缺血后血脑屏障的保护作用
The Protective Effects of Soluble Epoxidehydrolase Inhibitor TPPU on Blood Brain Barrier following Cerebral Ischemia in Rats
摘要
Abstract
Objective:To investigate the effect of soluble epoxidehydrolase inhibitor (sEHi) TPPU on blood brain barrier (BBB) function in rats after focal ischemia.Methods:Rat focal cerebral ischemia model was established by transient middle cerebral artery occlusion (MCAO) via intraluminal suture.Forty-five male SD rats were randomly divided into three groups:the sham group,MCAO group and TPPU group (n=15 respectively).Neurologic deficits and behavioral function recovery were assessed by Garcia neurological severity score.The degree of cerebral edema was evaluated by dry/wet weight method.The infarct volume was evaluated by TTC staining.BBB permeability was determined by detecting the leakage of Evens Blue (EB) dye in the brain tissue.Immunofluorescent staining and western blotting were used to observe the content changes of tight junction proteins of BBB.Results:Compared with those in the MCAO group,the treatment of TPPU helped to improve the neurological deficits,decrease the infarct volume of cerebral cortex,reduce the vasogenic brain edema,decrease the leakage of EB in brain tissue and alleviate the loss of tight junction proteins in BBB (P＜0.05).Conclusion:TPPU plays an active role in protecting the BBB integrity,mitigating cerebral edema,decreasing infarct volume and ameliorating the neurological deficit in rats after MCAO.关键词
TPPU/血脑屏障/紧密连接蛋白/脑缺血Key words
TPPU/blood-brain barrier/tight junction protein/cerebral ischemia分类
医药卫生引用本文复制引用
胡晓静,张贵斌,谢敏杰..可溶性环氧化物水解酶抑制剂TPPU对大鼠局灶脑缺血后血脑屏障的保护作用[J].神经损伤与功能重建,2017,12(4):286-289,4.基金项目
国家自然科学基金(No.81371312) （No.81371312）
