南方农业学报2017，Vol.48Issue(7)：1148-1154,7.DOI:10.3969/j.issn.2095-1191.2017.07.03
茄子SSR多态性标记筛选及杂交种纯度鉴定
Screening of polymorphic SSR markers and hybrid seed purity identification of Solanum melongena L.
摘要
Abstract
[Objective]A fast, accurate and effective method for testing purity of Solanum melongena L. hybrids was established to provide technical support for testing the purity in seed production and promoting its application in large scale.[Method]Forty-eight released SSR primers were selected for polymorphisms screening of parents of two eggplant hybrids 15-16 and 1-7. Then, effective primers were selected to identify the purity of S. melongena L. hybrids seeds. Combining field plant purity identification, the validity of purity identification by SSR molecular markers was verified. [Result]Among the 48 pairs of primers, 12 pairs showed polymorphism in parent of hybrid 15-16, in which polymorphic primers banding type of 8 pairs was complementary type(SM14, SM15, SM17, SM20, SM29, SM30, SM34 and SM45). Five pairs showed polymorphism in parent of hybrid 1-7, in which polymorphic primers banding type of four pairs was complemen-tary type(SM15, SM20, SM24 and SM29). Two pairs of SSR primers which were complementary types to each other were selected for hybrid purity identification. The results indicated that the purities of hybrid 15-16 and hybrid 1-7 were 99% and 100% respectively, which were in line with field identification. [Conclusion]Purity identification of S. melongena L. hybrids seeds by SSR molecular markers is accurate and effective, which can provide technical support in identifying purity of hy-brid seed production.关键词
茄子/杂交种/纯度鉴定/SSR分子标记Key words
Solanum melongena L./hybrid/purity identification/SSR molecular marker分类
农业科技引用本文复制引用
张洪源,谈杰,张敏,黄树苹,陈霞,王春丽,谈太明..茄子SSR多态性标记筛选及杂交种纯度鉴定[J].南方农业学报,2017,48(7):1148-1154,7.基金项目
国家科技支撑计划项目(2012BAD02B02) （2012BAD02B02）
湖北省科技支撑计划项目(2015BBA202) （2015BBA202）
