南方农业学报2017，Vol.48Issue(7)：1173-1177,5.DOI:10.3969/j.issn.2095-1191.2017.07.07
油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响
Effects of brassinolide on growth morphology and physiological characters of Avena nuda under room and high temperature stresses
摘要
Abstract
[Objective]The regulation mechanism of brassinolide on growth and antioxidant metabolism of Avena nuda was explored,so as to provide some references for improving productivity and heat resistance of A. nuda. [Method]The ex-periment set up the treatment of spraying distilled water as control(CK). Brassinolide of different concentrations(0.01,0.10 and 1.00 mg/L) were sprayed on leaves of A. nuda under room temperature(day 20 ℃/night 15 ℃) and high temperature (day 38 ℃/night 25 ℃) stresses to explore the effects of brassinolide on morphological indexes and physiological and bio-chemical indexes after 7 d. [Result]Under room temperature, spraying brassinolide increased chlorophyll content in A. nuda, strengthened photosynthesis and increased biomass accumulation. Plant height,fresh and dry weight of A. nuda were the highest in 1.00 mg/L brassinolide and were 23%, 26% and 123% higher than those of CK. Chlorophyll a,chlorophyll b and total chlorophyll contents of A. nuda treated by 0.10 mg/L were the highest and 81%, 98% and 86% higher than those of CK. Brassinolide significantly improved heat resistance under high temperature by increasing the activity of root system and antioxidant enzyme activity, reducing malondialdehyde (MDA ) content. In 0.10 mg/L brassinolide treatment group , root activity was the highest (154.16 μg/g·h)and MDA content was the lowest(17.54 nmol/g). Soluble protein content (28.75 mg/g),soluble sugar content (26.75 mg/g),superoxide dismutase (SOD) activity (842.71 U/gFW) and cata-lase (CAT) activity (199.43 U/g·min) reached the maximum. [Conclusion]Under room temperature, spraying 0.10 and 1.00 mg/L brassinolide has the optimal improving effects on growth of A. nuda. Under high temperature stress, spray-ing 0.10 mg/L brassinolide has the best effects on improving heat resistance of A. nuda.关键词
裸燕麦/油菜素内酯/高温胁迫/生长形态/光合色素/抗氧化酶Key words
Avena nuda/brassinolide/high temperature stress/growth morphology/photosynthetic pigment/an-tioxidant enzyme分类
农业科技引用本文复制引用
王传凯,郭淼..油菜素内酯对裸燕麦常温和高温胁迫下生长形态及生理特性的影响[J].南方农业学报,2017,48(7):1173-1177,5.基金项目
国家自然科学基金项目(30971704) （30971704）
