南方农业学报2017，Vol.48Issue(7)：1220-1224,5.DOI:10.3969/j.issn.2095-1191.2017.07.15
贵州辣椒脉斑驳病毒的检测及株系分化研究
Detection of Chilli veinal mottle virus from Guizhou and its strain differentiation
摘要
Abstract
[Objective]The present study aimed to define the distribution and evolution of Chilli veinal mottle virus (ChiVMV) in Guizhou, and provide scientific evidence for the interaction between gene function of the virus and host plant. [Method]The reverse transcription PCR method(RT-PCR) was used to amplify the suspected ChiVMV pepper sam-ples that collected from Guiyang, Zunyi, Qianxi and Dafang. Subsequently, the PCR products were amplified and se-quenced. Sequence splicing and analysis of ChiVMV CP gene and 3'-UTR were performed by DNAMAN, and the phyloge-netic tree was established by MEGA. [Result]Seven positive samples were detected from thirty-nine samples of suspected ChiVMV peppers, the detection rate was 17.95%. Partial CP and 3'-UTR sequences of ChiVMV strain in Guizhou were compared with nucleotide sequence in NCBI. They showed the highest homology with the ChiVMV strain from Sichuan pepper, which was 99%. And their homology with ChiVMV strain in other provinces were above 90%. The phylogenetic tree also showed that the relationship between Guizhou strains, Sichuan strains, Yunnan strains were the closest. [Conclu-sion]ChiVMV strains have obvious plant differentiation in Guizhou.关键词
辣椒/辣椒脉斑驳病毒/株系分化/贵州Key words
pepper/Chilli veinal mottle virus/strain differentiation/Guizhou分类
农业科技引用本文复制引用
王莉爽,陈小均,何海永,谭清群,陈文,黄露,杨学辉..贵州辣椒脉斑驳病毒的检测及株系分化研究[J].南方农业学报,2017,48(7):1220-1224,5.基金项目
公益性行业(农业)科研专项项目(201303028) （农业）
贵州省农业科学院自主创新科研专项项目(2014002) （2014002）
