南方农业学报2017，Vol.48Issue(7)：1274-1279,6.DOI:10.3969/j.issn.2095-1191.2017.07.24
南美蟛蜞菊叶蛋白提取方法筛选
Evaluation of leaf protein extraction methods from Wedelia trilobata
摘要
Abstract
[Objective]The leaf protein extraction method and conditions from Wedelia trilobata were selected and op-timized, which provided technical support for the exploitation and utilization of W. trilobata as protein feed resources.[Method]Using orthogonal experiment,four leaf protein extraction methods including acid heating method,alkali heating method, salting out method and organic solvent method were optimized and extraction results were compared.Hence the optimal extraction conditions from W. trilobata were determined. [Result]According to the extracting leaf crude protein content from W. trilobata,the four methods were ranked as follows:salting out method (15.18 mg/g)>acid heating method(9.97 mg/g)>alkaline heating method(9.79 mg/g)>organic solvent method(7.21 mg/g). The optimum extraction conditions for salting out method were as follows:concentration of sodium chloride solution was 30%,flocculation time was 10 min. For acid heating method,the optimum extraction conditions were flocculation temperature 60 ℃,pH 3 and floccula-tion time 2 min. The optimum conditions for alkaline heating method were flocculation temperature 25 ℃,pH 7 and floccu-lation time 2 min. The optimum conditions for organic solvent method were 20% ethanol volume fraction and 10 min for flocculation. Flocculation temperature had significant effect on extraction efficiency for acid heating method and alkali heat-ing method(P<0.05),and concentration of extracting solution had extremely significant effect on extraction efficiency for salting out method and organic solvent method(P<0.01). [Conclusion]By comparing the four methods,salting out method was determined as the optimal method for extracting leaf protein from W. trilibata because of its high extraction efficiency, simple operation,high protein activity which enabling it suitable for laboratory extraction and industrial production.关键词
南美蟛蜞菊/叶蛋白/提取方法Key words
Wedelia trilobata/leaf protein/extraction method分类
农业科技引用本文复制引用
陈秀清,胡亦平,林益坤,刘昭弟,宋莉英..南美蟛蜞菊叶蛋白提取方法筛选[J].南方农业学报,2017,48(7):1274-1279,6.基金项目
广东省自然科学基金项目(S2012010010027) （S2012010010027）
广东省教育厅高等学校优秀青年教师培养计划项目(Yq2013132) （Yq2013132）
广州市科技计划项目(201707010257) （201707010257）
广州大学大学生创新训练项目(201611078061) （201611078061）
