海南医学院学报2017，Vol.23Issue(14)：1903-1906,4.DOI:10.13210/j.cnki.jhmu.20170809.014
溃疡性结肠炎患者肠道菌群紊乱与TLR/NK-kB、炎症介质表达的关系
Relationship of intestinal flora disorder with TLR/NK-kB and inflammatory mediator expression in patients with ulcerative colitis
摘要
Abstract
Objective: To study the relationship of intestinal flora disorder with TLR/NK-kB and inflammatory mediator expression in patients with ulcerative colitis (UC).Methods: Patients who were diagnosed with UC in Yan'an People's Hospital were selected, patients with active UC and patients with remission UC were included as the AUC and RUC group;patients who were diagnosed with colonic polyps in Yan'an People's Hospital through physical examination during the same period were selected as the control group.Feces was collected to test the flora number, mucosa lesion was collected to determine the expression of TLR/NK-kB and inflammatory mediators, and serum was collected to detect the levels of inflammatory mediators.Results: Bifidobacterium bifidum, Lactobacillus acidophilus and bacteroides counts in feces of AUC group and RUC group were significantly lower than those of control group while Escherichia coli and Escherichia enterococcus number in feces, TLR2, TLR4, TLR5, TLR9, NK-kB, HMGB1, TNF-α, IL-6 and IL-17 mRNA expression in intestinal mucosa lesion as well as HMGB1, TNF-α, IL-6 and IL-17 levels in serum were significantly higher than those of control group;Bifidobacterium bifidum, Lactobacillus acidophilus and bacteroides counts in feces of AUC group were significantly lower than those of RUC group while Escherichia coli and Escherichia enterococcus number in feces, TLR2, TLR4, TLR5, TLR9, NK-kB, HMGB1, TNF-α, IL-6 and IL-17 mRNA expression in intestinal mucosa lesion as well as HMGB1, TNF-α, IL-6 and IL-17 levels in serum were significantly higher than those of RUC group;TLR2, TLR4, TLR5, TLR9, NK-kB, HMGB1, TNF-α, IL-6 and IL-17 mRNA expression in intestinal mucosa lesion as well as HMGB1, TNF-α, IL-6 and IL-17 levels in serum were negatively correlated Bifidobacterium bifidum, Lactobacillus acidophilus and bacteroides counts, but positively correlated with Escherichia coli and Escherichia enterococcus counts.Conclusion: The intestinal flora disorder in patients with ulcerative colitis can increase the expression of inflammatory mediators by activating the TLR/NK-kB pathway.关键词
溃疡性结肠炎/肠道菌群紊乱/Toll样受体/炎症介质Key words
Ulcerative colitis/Intestinal flora disorder/Toll-like receptor/Inflammatory mediator分类
医药卫生引用本文复制引用
马涛..溃疡性结肠炎患者肠道菌群紊乱与TLR/NK-kB、炎症介质表达的关系[J].海南医学院学报,2017,23(14):1903-1906,4.基金项目
陕西省科学技术厅项目(9612013Y0286)This study was supported by Shaanxi Science and Technology Department project (9612013Y0286). （9612013Y0286）
