华西口腔医学杂志2017，Vol.35Issue(4)：362-367,6.DOI:10.7518/hxkq.2017.04.004
不同体积分数氧气对山羊颞下颌关节盘细胞骨架改建的影响
Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells
摘要
Abstract
Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O2) and hypoxia (2%, 4%, and 8% O2). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse trans-cription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O2 (P<0.05), whereas that of tubulin was highest at 2% O2 (P<0.05). No significant difference among the other groups was observed (P>0.05). Actin mRNA levels were considerably decreased at 2% O2 and 4% O2 in hypoxic conditions, while actin mRNA expression was highest in 21% O2. Tubulin mRNA levels considerably increased at 2% O2, while tubulin mRNA expression was lowest in 8% O2 (P<0.05).Vimentin mRNA expression was lowest at 4% O2 and highest at 21% O2, and significant differences were observed between vimentin mRNA expression levels among these oxygen levels (P<0.05). Conclusion Cytoskeletons were recon-structed in different oxygen tensions, and 2% O2 may be the optimal oxygen level required to proliferate TMJ disc cells.关键词
颞下颌关节盘/低氧/细胞骨架/肌动蛋白/微管蛋白/波形蛋白Key words
temporomandibular joint disc/hypoxia/cytoskeleton/actin/tubulin/vimentin分类
医药卫生引用本文复制引用
何晓兰,包广洁,孙凌璐,张雪,保善英,康宏..不同体积分数氧气对山羊颞下颌关节盘细胞骨架改建的影响[J].华西口腔医学杂志,2017,35(4):362-367,6.基金项目
国家自然科学基金(81160139,81660189) （81160139,81660189）
Natural Nation Science Foundation of China (81160139, 81660189). （81160139, 81660189）
