华西口腔医学杂志2017，Vol.35Issue(4)：373-378,6.DOI:10.7518/hxkq.2017.04.006
体外沉默异戊二烯化酶二牛龙牛儿基转移酶Ⅰ对舌鳞状细胞癌增殖的影响
Effects of geranylgeranyltransferaseⅠsilencing on the proliferation of tongue squamous cancer cells
摘要
Abstract
Objective This study aims to investigate the effect of geranylgeranyltransferaseⅠ (GGTase-Ⅰ) on the proliferation and growth of tongue squamous cancer cells. Methods Three small interfering RNAs (siRNAs) were designed on the basis of the GGTase-Ⅰ sequence in GeneBank. These siRNAs were then transfected into tongue squamous cancer cells Cal-27. The mRNA and protein expression of GGTase-Ⅰ and RhoA were examined by real-time quantitative polymerase chain reaction and Western blotting, respectively. The expression of Cyclin D1 and p21 were examined by Western blotting. The proliferation and growth ability were analyzed by cell counting kit-8 assay and flow cytometry. Results The mRNA and protein expression of GGTase-Ⅰ in Cal-27 was reduced significantly after the GGTase-Ⅰ siRNAs were transfected (P<0.05). No significant difference in RhoA mRNA and protein expression was detected (P>0.05). Cyclin D1 expression decreased, whereas p21 expression increased significantly. The cell cycle was altered, and the growth-proliferative activity was inhibited (P<0.05). Conclusion GGTase-Ⅰ siRNA can inhibit the expression of GGTase-Ⅰ and the proliferative activity of tongue squamous cancer cells. GGTase-Ⅰ may be a potential target for gene therapy in tongue squamous cell cancer.关键词
异戊二烯化酶二牛龙牛儿基转移酶Ⅰ/RhoA/舌鳞状细胞癌/增殖/CyclinD1/p21Key words
geranylgeranyltransferaseⅠ/RhoA/tongue squamous cancer/proliferation/Cyclin D1/p21分类
医药卫生引用本文复制引用
王莹,周元,廖奕翔,何宗轩,李宁,曹蕾,刘文君,陈正岗,王奇民,李敬华,韩金宏,王莉莉,巢晨,周建华,童磊,鲁旭飞..体外沉默异戊二烯化酶二牛龙牛儿基转移酶Ⅰ对舌鳞状细胞癌增殖的影响[J].华西口腔医学杂志,2017,35(4):373-378,6.基金项目
国家自然科学基金(81372908) （81372908）
青岛市卫计委计划项目(2014-WJZD009,2013-WSZD011)The National Natural Science Foundation of China (81372908) （2014-WJZD009,2013-WSZD011）
Project of Qingdao Municipal Health and Family Planning Commission (2014-WJZD009, 2013-WSZD011). （2014-WJZD009, 2013-WSZD011）
