渔业科学进展2017，Vol.38Issue(4)：53-60,8.DOI:10.11758/yykxjz.20160325001
干露胁迫对脊尾白虾(Exopalaemon carinicauda)呼吸代谢相关酶的影响
Effects of Dry Exposure on the Respiratory Metabolism-Related Enzymes of Exopalaemon carinicauda
摘要
Abstract
The objective of the present research was to investigate the effects of dry exposure on respiratory metabolism in different tissues of Exopalaemon carinicauda.In the trial,E.carinicauda were divided into two experimental groups,namely normal temperature (22-24℃) group and low temperature (4℃) group.Meanwhile,shrimp cultured in seawater were used as the control group.Activities of respiratory metabolism enzymes and content of lactate were measured under dry exposure challenges at 60 min,90 min,120 min and 180 min.The investigated enzymes included succinate dehydrogenase (SDH),cytochrome oxidase (CCO),fumarate reductase (FRD) and lactate dehydrogenase (LDH).SDH and CCO are the key enzymes of aerobic metabolism in animals,whereas FRD and LDH are the key enzymes of anaerobic metabolism in animals.In addition,the content of lactate is an indicator of anaerobic metabolism.The results showed that the activities of SDH and CCO in hepatopancreas,gill,and muscle in the normal temperature group decreased significantly than those in the control (P＜0.05) with the processes of dry exposure.Activities of FRD,LDH and content of lactate in muscle increased initially and then decreased gradually.And the levels were significantly higher than those in the control (P＜0.05).Activities of respiratory metabolism enzymes and content of lactate in low temperature group had no significant difference compared to those in the control group at 60 min.After that,the trends of the enzyme levels in the low temperature group were as similar as the normal temperature group.The changes of enzyme activities were tissue dependent.Activities of respiratory metabolic enzymes in hepatopancreas and gill were higher than those in muscle.The results suggested that anaerobic metabolism increased after dry exposure.By contrast,E.carinicauda can maintain the aerobic metabolism during the early stage of low temperature dry exposure.Furthermore,the hepatopancreas and gill might be more sensitive to dry exposure,whereas reduced temperature could increase the survival rate ofE.carinicauda.关键词
脊尾白虾/干露/呼吸代谢酶/无氧代谢Key words
Exopalaemon carinicauda/Dry exposure/Respiratory metabolism enzymes/Anaerobic metabolism分类
农业科技引用本文复制引用
连春盎,李健,李吉涛,冯艳艳..干露胁迫对脊尾白虾(Exopalaemon carinicauda)呼吸代谢相关酶的影响[J].渔业科学进展,2017,38(4):53-60,8.基金项目
国家虾产业技术体系(CARS-47)、山东省泰山产业领军人才工程项目(LNJY2015002)、国家自然科学基金项目(31472275)、青岛市产业培育计划科技惠民专项(17-3-3-62-nsh)、中国水产科学研究院黄海水产研究所基本科研业务费专项资金项目(20603022016009)和宁波市科技计划项目(2016C11011)共同资助[This work was supported by the Earmarked Fund for Modem Agro-Industry Technology Research System (CARS-47),the Program of Shandong Leading Talent (LNJY2015002),National Natural Science Foundation of China (31472275),Qingdao Industrial Development Program Science and Technology Benefit Special Project (17-3-3-62-nsh),Special Scientific Research Funds for Central Non-Profit Institutes,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences (20603022016009),and Projects of Science and Technology of Ningbo (2016C11011)]. （CARS-47）
