基础医学与临床2017，Vol.37Issue(7)：918-922,5.
辣椒素诱导人骨肉瘤细胞MG-63发生免疫原性细胞死亡
Immunogenic cell death of human osteosarcoma cells MG-63 induced by capsaicin
摘要
Abstract
Objective To investigate the effect of capsaicin and cisplatin on the proliferation and immunogenic cell death of human osteosarcoma cells.Methods MTT assay was used to examine the growth inhibiting effects of capsacin and cisplation on MG-63 cells;Mitochondrial membrane potential (MMP) was used to investigate the apoptosis;flow cytometry was used to detect the expression of calreticulin (CRT) on the cell membrane,fluorescein enzymatic method was used to detect the release of ATP,ELISA was used to detect the secretion of high mobility group B1 (HMGB1).Results Capsaicin and cisplatin can inhibit MG-63 cells proliferation in the dose-dependent manner and induce MG-63 apoptosis(P＜0.01).Only capsaicin can induce translocation of CRT from endoplasmic reticulum to the cell surface and release of extracellular ATP and HMGB1 (P＜0.01).Conclusions Capsaicin can induce human osteosarcoma cells apoptosis and immunogenic cell death.关键词
辣椒素/免疫原性细胞死亡/骨肉瘤Key words
capsaicin/immunogenic cell death/osteosarcoma分类
医药卫生引用本文复制引用
金涛,彭昊,王艳林,吴红艳..辣椒素诱导人骨肉瘤细胞MG-63发生免疫原性细胞死亡[J].基础医学与临床,2017,37(7):918-922,5.基金项目
国家自然科学基金(81402557) （81402557）
