基础医学与临床2017，Vol.37Issue(7)：935-938,4.
针对于白蛋白的新型核酸适配体的筛选与鉴定
Screening and identification of a novel DNA aptamer against albumin
摘要
Abstract
Objective To develop an albumin aptamer that may potentially serve as a selective ligand for albumin removal from experimental samples.Methods A single-stranded 59nt DNA library that contains 21 random oligo nucleotides was synthesized in vitro.An albumin aptamer A6 was developed by SELEX technique using bovine serum albumin (BSA) as target.The enrichment of aptamer and evaluation of its binding properties were monitored by flow cytometry.The secondary structure of A6 was predicted by MFord software.Results The aptamer A6 strongly bound to BSA with a Kd of 77.4 nmol/L,and had minimal cross reactivity with control proteins including ovalbu min,IgG,and trypsin.Conclusions Aptamer A6 may be a potential tool in albumin removal.关键词
核酸适配体/SELEX/白蛋白Key words
aptamer/SELEX/albumin分类
医药卫生引用本文复制引用
李晓欧,胡燕,安雅聪,段金虹,杨先达..针对于白蛋白的新型核酸适配体的筛选与鉴定[J].基础医学与临床,2017,37(7):935-938,4.基金项目
国家自然科学基金(81572997) （81572997）
