基础医学与临床2017，Vol.37Issue(7)：953-958,6.
HIV-1早期感染者γδT细胞对储藏库细胞的细胞毒作用
Cytotoxic role of γδT cells to latency cells in patients with early human immunodeficiency virus-1 infection
摘要
Abstract
Objective To investigate the cytotoxicity of γδ T cells to HIV-1 latency cells in patients with early HIV-1 infection.Methods Sixteen early HIV-1-infected patients were enrolled in this study.Peripheral blood mononuclear cells (PBMCs) of patients were isolated and γδ T cells were expanded using zoledronate (5 μmol/L) and interleukin (IL)-2 (1 000 IU/mL) ex vivo.Lactic dehydrogenase (LDH) was used to detect the cytotoxic role of γδ T cells to HIV-1 latency cells(J-Lat Full Length Clonel0.6).The phenotype of γδ T cells before and after expansion and the intensity of GFP in HIV-1 latency cells were detected by flow cytometry.Results Zoledronate plus IL-2 stimulated rapid and large γδ T cells proliferation ex vivo (P＜0.001).γδ T cells showed high cytotoxici ty to latency cells,and the intensity of GFP in latency cells was decreased significantly (P＜0.05).Moreover,expanded γδ T cells displayed cytotoxic NK-like phenotype,the frequency of CD56+ Vδ2 T cells in patients with early HIV-1 infection was significantly higher than that of healthy controls.Conclusions γδ T cell has an ability to eradicate HIV-1 latency,and γδ T cell-based autologous or xenogenous adoptive immunotherapy will have promise prospects to cure HIV-1 infection.关键词
人类免疫缺陷病毒-1/储藏库/γδ T细胞/细胞治疗Key words
human immunodeficiency virus-1/latency/γδ T cell/immunotherapy分类
医药卫生引用本文复制引用
李珍,陆小凡,孙坚萍,粟斌,吴昊,张永宏,张彤..HIV-1早期感染者γδT细胞对储藏库细胞的细胞毒作用[J].基础医学与临床,2017,37(7):953-958,6.基金项目
国家自然科学基金青年基金(81501731) （81501731）
北京市艾滋病重点实验室项目(BZ0089) （BZ0089）
北京市科技计划项目(D141100000314005) （D141100000314005）
