基础医学与临床2017，Vol.37Issue(8)：1103-1107,5.
硬膜外罗哌卡因阻滞加强丙泊酚静脉麻醉的深度
Epidural ropivacaine block increasing anesthetic potency of propofol
摘要
Abstract
Objective To investigate the effects of epidural ropivacaine block combined with propofol intravenous anesthesia on CaMKⅡ and ERK1/2 total protein (T-CaMKⅡ and T-ERK1/2) and phosphorylation(p-CaMKⅡ and p-ERK1/2) levels in the hippocampus and cortex of rats.Methods Rats were randomly assigned to three groups: group P(control,propofol intravenous anesthesia),group PS(propofol and epidural normal saline) and group PR(propofol and epidural 0.5% ropivacaine).Anesthesia were performed in 72 h after epidural catheter placement.The rats in group PR received 70 μL of 0.5% ropivacaine to achieve epidural block.1% propofol was infused through rats caudal vein.Propofol dosage for anesthesia induction was 12 mg/kg,for anesthesia maintenance was 40 mg/(kg·h).Before the rats were decapitated,the depth of anaesthesia was assessed as either "light anesthesia" or "deep anesthesia" by checking of pinch withdrawalreflex,eyelid reflex and spontaneous rapid whisking of the vibrissae after propofol continuous infusion for 1 h.T-CaMKⅡ/T-ERK1/2 and p-CaMKⅡ/p-ERK1/2 in hippocampus and frontal cortex were examined by Western blot.Results 7 rats were assessed as "light anesthesia" and one rat as "deep anesthesia" in group P;6 rats were assessed as "light anesthesia" and 2 rats as "deep anesthesia" in group PS;in group PR,1 rat was assessed as "light anesthesia" and 7 rats as "deep anesthesia".Significant differences were seen among three groups (P<0.05).In hippocampus of rats,p-CaMKⅡ(Thr286)43.7%±8.8% and p-ERK1/2 32.4%±7.9% in group PR were significantly lower than those in group P (100%,P<0.05).Conclusions Epidural ropivacaine block may strengthen the depth of anesthesia achieved with propofol intravenous anesthesia.The decrease of p-CaMKⅡ(Thr286) and p-ERK1/2 in hippocampus of rats may explain the effects of epidural block.关键词
丙泊酚/硬膜外阻滞/CaMKⅡ/ERK1/2Key words
propofol/epidural block/CaMKⅡ/ERK1/2分类
医药卫生引用本文复制引用
崔旭,赵晓艳,张炳熙,潘楚雄,李俊发..硬膜外罗哌卡因阻滞加强丙泊酚静脉麻醉的深度[J].基础医学与临床,2017,37(8):1103-1107,5.基金项目
北京市优秀人才培养资助计划(2013D003034000031) （2013D003034000031）
